Linked Life Data

10063940

Source:http://linkedlifedata.com/resource/pubmed/id/10063940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-16
pubmed:abstractText
Safety and thrombus imaging capabilities of the 99mTc-labeled form of a new F(ab')2 monoclonal antibody (MoAb) against fragment D dimers from cross-linked human fibrin, previously shown to be effective labeled to 131I in detecting venous thrombi in the rabbit, were investigated. Sixteen patients (seven men, mean age: 60+/-7 years) with deep (n = three) and superficial (n = 13) venous thromboses of the lower limbs documented at echo-Doppler study underwent, 24 hours before saphenous vein stripping, a scintigraphic study after IV injection of the 99mTc-MoAb (1,129+/-275 MBq/mL), acquiring dynamic images, as well as early and delayed static images of lower limbs. Tracer activity was compared in normal and pathologic areas. At the operation, vessel wall including the thrombotic lesion was isolated, weighed, and counted. Blood radioactivity and MoAb concentration were also measured. No adverse reaction was observed after MoAb administration. Thrombus site appeared as a focal area (hot spot) of asymmetrically increased tracer uptake, already detectable at early images in all patients. All thrombi detected at echo-Doppler study (n=25) were confirmed at scintigraphic study, which showed four additional hot spots subsequently confirmed to represent thrombi at operation. Average percent ratio between pathologic and normal regions was 1.51+/-1.34 (p < 0.05) at time-activity curves, 2.27+/-1.1 (p < 0.05) at early static images, and 2.15+/-1.2 (p < 0.05) at delayed images, respectively. Thrombus-to-blood uptake ratio was 4.3+/-0.9 (p < 0.01). The F(ab')2 MoAb proved to be safe, and low levels of antimouse antibodies were detected in response, although further studies are needed to assess tolerance and effectiveness in case of a second administration in the same patient. The 99mTc-labeled MoAb was very effective in identifying venous thromboses both at deep and superficial localizations, although its sensitivity and specificity need be evaluated in a more numerous group, including also patients with different and clinically more relevant localizations, such as caval thromboses. However, the possibility of obtaining high-quality images within 4 hours of MoAb administration is clinically relevant, and carries also therapeutic implications, especially in pulmonary thromboembolism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-3197
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10063940-Antibodies, Monoclonal, pubmed-meshheading:10063940-Female, pubmed-meshheading:10063940-Fibrin Fibrinogen Degradation Products, pubmed-meshheading:10063940-Humans, pubmed-meshheading:10063940-Immunoglobulin Fab Fragments, pubmed-meshheading:10063940-Leg, pubmed-meshheading:10063940-Male, pubmed-meshheading:10063940-Middle Aged, pubmed-meshheading:10063940-Pulmonary Embolism, pubmed-meshheading:10063940-Radioimmunodetection, pubmed-meshheading:10063940-Radiopharmaceuticals, pubmed-meshheading:10063940-Safety, pubmed-meshheading:10063940-Saphenous Vein, pubmed-meshheading:10063940-Sensitivity and Specificity, pubmed-meshheading:10063940-Technetium, pubmed-meshheading:10063940-Thrombectomy, pubmed-meshheading:10063940-Ultrasonography, Doppler, pubmed-meshheading:10063940-Venae Cavae, pubmed-meshheading:10063940-Venous Thrombosis
pubmed:year
1999
pubmed:articleTitle
Immunoscintigraphy of venous thrombi: clinical effectiveness of a new antifibrin D-dimer monoclonal antibody.
pubmed:affiliation
Department of Experimental Medicine, University La Sapienza, Rome, Italy.
pubmed:publicationType
Journal Article, Comparative Study