Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-4-15
pubmed:abstractText
Fibronectin (FN) forms the primitive fibrillar matrix in both embryos and healing wounds. To study the matrix in living cell cultures, we have constructed a cell line that secretes FN molecules chimeric with green fluorescent protein. These FN-green fluorescent protein molecules were assembled into a typical matrix that was easily visualized by fluorescence over periods of several hours. FN fibrils remained mostly straight, and they were seen to extend and contract to accommodate movements of the cells, indicating that they are elastic. When fibrils were broken or detached from cells, they contracted to less than one-fourth of their extended length, demonstrating that they are highly stretched in the living culture. Previous work from other laboratories has suggested that cryptic sites for FN assembly may be exposed by tension on FN. Our results show directly that FN matrix fibrils are not only under tension but are also highly stretched. This stretched state of FN is an obvious candidate for exposing the cryptic assembly sites.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-1544488, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-2045422, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-222466, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-6643500, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-667946, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-7867709, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-7937847, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8034677, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8114919, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8501121, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8548820, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8813154, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-8999851, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9148804, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9148805, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9153398, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9217327, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9472037, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9548730, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9580564, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9603523, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9630892, http://linkedlifedata.com/resource/pubmed/commentcorrection/10051610-9822581
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2153-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein.
pubmed:affiliation
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710-3011, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.