pubmed-article:10051434 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1332794 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C0458827 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C0031621 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C0752312 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1414253 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1424650 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1539079 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
pubmed-article:10051434 | lifeskim:mentions | umls-concept:C0074992 | lld:lifeskim |
pubmed-article:10051434 | pubmed:dateCreated | 1999-5-4 | lld:pubmed |
pubmed-article:10051434 | pubmed:abstractText | We report here that cultured airway smooth muscle cells contain transcripts of endothelial differentiation gene 1 (EDG-1), a prototypical orphan Gi-coupled receptor whose natural ligand is sphingosine 1-phosphate (S1P). This is consistent with data that showed that S1P activated both c-Src and p42/p44 mitogen-activated protein kinase (p42/p44 MAPK) in a pertussis toxin (PTX)-sensitive manner in these cells. An essential role for c-Src was confirmed by using the c-Src inhibitor, PP1, which markedly decreased p42/p44 MAPK activation. We have also shown that phosphoinositide 3-kinase (PI-3K) inhibitors (wortmannin and LY294002) decreased p42/p44 MAPK activation. An essential role for PI-3K was supported by experiments that showed that PI-3K activity was increased in Grb-2 immunoprecipitates from S1P-stimulated cells. Significantly, Grb-2 associated PI-3K activity was decreased by pretreatment of cells with PTX. Finally, we have shown that the co-stimulation of cells with platelet-derived growth factor (PDGF) and S1P (which failed to stimulate DNA synthesis) elicited a larger p42/p44 MAPK activation over a 30 min stimulation compared with each agonist alone. This was associated with a S1P-dependent increase in PDGF-stimulated DNA synthesis. These results demonstrate that S1P activates c-Src and Grb-2-PI-3K (intermediates in the p42/p44 MAPK cascade) via a PTX-sensitive mechanism. This action of S1P is consistent with the stimulation of EDG-1 receptors. S1P might also function as a co-mitogen with PDGF, producing a more robust activation of a common permissive signal transduction pathway linked to DNA synthesis. | lld:pubmed |
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pubmed-article:10051434 | pubmed:language | eng | lld:pubmed |
pubmed-article:10051434 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10051434 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:10051434 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:10051434 | pubmed:month | Mar | lld:pubmed |
pubmed-article:10051434 | pubmed:issn | 0264-6021 | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:BowenPP | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:TateRR | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:RakhitSS | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:PyneN JNJ | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:PyneSS | lld:pubmed |
pubmed-article:10051434 | pubmed:author | pubmed-author:ConwayA MAM | lld:pubmed |
pubmed-article:10051434 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:10051434 | pubmed:day | 15 | lld:pubmed |
pubmed-article:10051434 | pubmed:volume | 338 ( Pt 3) | lld:pubmed |
pubmed-article:10051434 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:10051434 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:10051434 | pubmed:pagination | 643-9 | lld:pubmed |
pubmed-article:10051434 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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pubmed-article:10051434 | pubmed:meshHeading | pubmed-meshheading:10051434... | lld:pubmed |
pubmed-article:10051434 | pubmed:year | 1999 | lld:pubmed |
pubmed-article:10051434 | pubmed:articleTitle | Sphingosine 1-phosphate stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle. Role of endothelial differentiation gene 1, c-Src tyrosine kinase and phosphoinositide 3-kinase. | lld:pubmed |
pubmed-article:10051434 | pubmed:affiliation | Department of Physiology and Pharmacology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 ONR, Scotland, UK. | lld:pubmed |
pubmed-article:10051434 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:10051434 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
entrez-gene:26417 | entrezgene:pubmed | pubmed-article:10051434 | lld:entrezgene |
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