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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6720
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pubmed:dateCreated |
1999-3-11
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pubmed:databankReference |
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pubmed:abstractText |
DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
397
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
579-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10050851-Amino Acid Sequence,
pubmed-meshheading:10050851-Animals,
pubmed-meshheading:10050851-Catalysis,
pubmed-meshheading:10050851-Cells, Cultured,
pubmed-meshheading:10050851-Cloning, Molecular,
pubmed-meshheading:10050851-Cytosine,
pubmed-meshheading:10050851-DNA,
pubmed-meshheading:10050851-DNA, Complementary,
pubmed-meshheading:10050851-DNA Methylation,
pubmed-meshheading:10050851-Dinucleoside Phosphates,
pubmed-meshheading:10050851-HeLa Cells,
pubmed-meshheading:10050851-Humans,
pubmed-meshheading:10050851-Methanol,
pubmed-meshheading:10050851-Mice,
pubmed-meshheading:10050851-Molecular Sequence Data,
pubmed-meshheading:10050851-Oxidoreductases, O-Demethylating,
pubmed-meshheading:10050851-Sequence Homology, Amino Acid,
pubmed-meshheading:10050851-Substrate Specificity,
pubmed-meshheading:10050851-Transfection,
pubmed-meshheading:10050851-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
A mammalian protein with specific demethylase activity for mCpG DNA.
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pubmed:affiliation |
Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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