rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2-3
|
pubmed:dateCreated |
1999-3-30
|
pubmed:abstractText |
Somatostatin inhibits glucagon-secretion from pancreatic alpha cells but its underlying mechanism is unknown. In mouse alpha cells, we found that somatostatin induced prominent hyperpolarization by activating a K+ channel, which was unaffected by tolbutamide but prevented by pre-treating the cells with pertussis toxin. The K+ channel was activated by intracellular GTP (with somatostatin), GTPgammaS or Gbetagamma subunits. It was thus identified as a G protein-gated K+ (K(G)) channel. RT-PCR and immunohistochemical analyses suggested the K(G) channel to be composed of Kir3.2c and Kir3.4. This study identified a novel ionic mechanism involved in somatostatin-inhibition of glucagon-secretion from pancreatic alpha cells.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/G Protein-Coupled...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Tolbutamide,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0014-5793
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
12
|
pubmed:volume |
444
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
265-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10050772-Acetylcholine,
pubmed-meshheading:10050772-Animals,
pubmed-meshheading:10050772-Electrophysiology,
pubmed-meshheading:10050772-Female,
pubmed-meshheading:10050772-G Protein-Coupled Inwardly-Rectifying Potassium Channels,
pubmed-meshheading:10050772-GTP-Binding Proteins,
pubmed-meshheading:10050772-Glucagon,
pubmed-meshheading:10050772-Guanosine Triphosphate,
pubmed-meshheading:10050772-Immunohistochemistry,
pubmed-meshheading:10050772-Ion Channel Gating,
pubmed-meshheading:10050772-Islets of Langerhans,
pubmed-meshheading:10050772-Mice,
pubmed-meshheading:10050772-Mice, Inbred Strains,
pubmed-meshheading:10050772-Patch-Clamp Techniques,
pubmed-meshheading:10050772-Pertussis Toxin,
pubmed-meshheading:10050772-Potassium Channels,
pubmed-meshheading:10050772-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:10050772-RNA, Messenger,
pubmed-meshheading:10050772-Somatostatin,
pubmed-meshheading:10050772-Tolbutamide,
pubmed-meshheading:10050772-Virulence Factors, Bordetella
|
pubmed:year |
1999
|
pubmed:articleTitle |
Somatostatin induces hyperpolarization in pancreatic islet alpha cells by activating a G protein-gated K+ channel.
|
pubmed:affiliation |
Department of Pharmacology II, Faculty of Medicine and Graduate School of Medicine, Osaka University, Suita, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|