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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0020663,
umls-concept:C0027882,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C1171362,
umls-concept:C1412082,
umls-concept:C1416589,
umls-concept:C1515670,
umls-concept:C1711351,
umls-concept:C1955862
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pubmed:dateCreated |
1999-4-27
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pubmed:abstractText |
1. Patch-clamp recordings were made from rat ventromedial hypothalamic neurones in slices of brain tissue in vitro. In cell-attached recordings, removal of extracellular glucose or metabolic inhibition with sodium azide reduced the firing rate of a subpopulation of cells through the activation of a 65 pS channel that was blocked by the sulphonylureas tolbutamide and glibenclamide. 2. In whole-cell patch-clamp recordings, in the absence of ATP in the electrode solution, glucose-receptive neurones gradually hyperpolarized due to the induction of an outward current at -60 mV. This outward current and the resultant hyperpolarization were blocked by the sulphonylureas tolbutamide and glibenclamide. 3. In recordings where the electrode solution contained 4 mM ATP, this outward current was not observed. Under these conditions, 500 microM diazoxide was found to induce an outward current that was blocked by tolbutamide. 4. In cell-attached recordings diazoxide and the active fragment of leptin (leptin 22-56) reduced the firing rate of glucose-receptive neurones by the activation of a channel with similar properties to that induced by removal of extracellular glucose. 5. Reverse transcription followed by the polymerase chain reaction using cytoplasm from single glucose-receptive neurones demonstrated the expression of the ATP-sensitive potassium (KATP) channel subunits Kir6.1 and SUR1 but not Kir6.2 or SUR2. 6. It is concluded that glucose-receptive neurones within the rat ventromedial hypothalamus exhibit a KATP channel current with pharmacological and molecular properties similar to those reported in other tissues.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-1348109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-14237464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-1467829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-1680516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2119205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2127550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2412077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2417160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2676059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-2988416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-3612561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-6322917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-7502040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-7509437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-7890693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-8004407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-8549751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9023770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9067450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9130167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9394003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9462882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9490811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9644047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9705995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10050011-9742245
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/sulfonylurea receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
515 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
439-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10050011-ATP-Binding Cassette Transporters,
pubmed-meshheading:10050011-Adenosine Triphosphate,
pubmed-meshheading:10050011-Animals,
pubmed-meshheading:10050011-Glucose,
pubmed-meshheading:10050011-Male,
pubmed-meshheading:10050011-Neurons,
pubmed-meshheading:10050011-Patch-Clamp Techniques,
pubmed-meshheading:10050011-Potassium Channels,
pubmed-meshheading:10050011-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:10050011-RNA, Messenger,
pubmed-meshheading:10050011-Rats,
pubmed-meshheading:10050011-Rats, Sprague-Dawley,
pubmed-meshheading:10050011-Receptors, Drug,
pubmed-meshheading:10050011-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10050011-Ventromedial Hypothalamic Nucleus
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pubmed:year |
1999
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pubmed:articleTitle |
Glucose-receptive neurones in the rat ventromedial hypothalamus express KATP channels composed of Kir6.1 and SUR1 subunits.
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pubmed:affiliation |
Parke Davis Neuroscience Research Centre, Cambridge University Forvie Site, Cambridge CB2 2QB, UK. kevin.lee@wl.com
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pubmed:publicationType |
Journal Article,
In Vitro
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