Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-4-1
pubmed:abstractText
MS-209 is a novel quinoline derivative reversing P-glycoprotein-mediated multidrug resistance (MDR). We investigated the interaction between MS-209 and multidrug resistance protein (MRP) in MRP-overexpressing human gastric cancer cells. We measured [3H]leukotriene C4 uptake into the membrane vesicles of the cells and intracellular calcein and [3H]vincristine accumulation with or without MS-209. In multi-drug-resistant MKN45R0.8 cells selected by doxorubicin, MS-209 dose dependently reduced MRP-mediated [3H]leukotriene C4 uptake and increased calcein accumulation. In both resistant and unselected cell lines expressing the MRP gene, MS-209 increased [3H]vincristine accumulation in proportion with the level of MRP mRNA expression and enhanced the cytotoxicity of etoposide, doxorubicin, and vincristine. The reversal effects correlated with the level of MRP mRNA expression in these cells. Our results indicate that MS-209 effectively reverses intrinsic and acquired MRP-mediated MDR of gastric cancer cells by interacting directly with MRP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters, http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin, http://linkedlifedata.com/resource/pubmed/chemical/Etoposide, http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins, http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene C4, http://linkedlifedata.com/resource/pubmed/chemical/Multidrug Resistance-Associated..., http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Probenecid, http://linkedlifedata.com/resource/pubmed/chemical/Quinolines, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Vincristine, http://linkedlifedata.com/resource/pubmed/chemical/dofequidar, http://linkedlifedata.com/resource/pubmed/chemical/fluorexon
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
255
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
618-24
pubmed:dateRevised
2006-5-20
pubmed:meshHeading
pubmed-meshheading:10049760-ATP-Binding Cassette Transporters, pubmed-meshheading:10049760-Antineoplastic Agents, pubmed-meshheading:10049760-Doxorubicin, pubmed-meshheading:10049760-Drug Resistance, Multiple, pubmed-meshheading:10049760-Etoposide, pubmed-meshheading:10049760-Fluoresceins, pubmed-meshheading:10049760-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10049760-Genes, MDR, pubmed-meshheading:10049760-Humans, pubmed-meshheading:10049760-Leukotriene C4, pubmed-meshheading:10049760-Multidrug Resistance-Associated Proteins, pubmed-meshheading:10049760-Neoplasm Proteins, pubmed-meshheading:10049760-Probenecid, pubmed-meshheading:10049760-Quinolines, pubmed-meshheading:10049760-RNA, Messenger, pubmed-meshheading:10049760-Stomach Neoplasms, pubmed-meshheading:10049760-Tumor Cells, Cultured, pubmed-meshheading:10049760-Vincristine
pubmed:year
1999
pubmed:articleTitle
Direct interaction between a quinoline derivative, MS-209, and multidrug resistance protein (MRP) in human gastric cancer cells.
pubmed:affiliation
Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, 852-8501, Japan.
pubmed:publicationType
Journal Article