Linked Life Data

10049517

Source:http://linkedlifedata.com/resource/pubmed/id/10049517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-4-5
pubmed:abstractText
Lymphotoxin alpha (LT-alpha) and lymphotoxin beta (LT-beta) are members of the tumour necrosis factor (TNF) ligand family. Because of the importance of TNF in the pathogenesis of septic shock, the expression of LT-alpha and LT-beta mRNA in murine splenocytes stimulated with different pro-inflammatory cytokines, sepsis-associated mediators such as lipopolysaccharide (LPS) and bacterial superantigens was investigated. The authors show that the bacterial superantigens, toxic shock syndrome toxin 1 (TSST-1) and staphylococcal enterotoxin B (SEB) upregulate LT-alpha mRNA expression in vitro in murine cells. Basal expression of LT-beta mRNA was found in unstimulated murine splenocytes, and could be increased by the addition of the mitogen concanavalin A (Con A). Despite this suggested inducibility of the murine LT-beta transcript, sepsis-associated mediators did not affect its regulation. Neither the pro-inflammatory cytokines interleukin 2 (IL-2), TNF-alpha nor LPS alone or in combination with interferon gamma (IFN-gamma) had any effect on LT-beta mRNA expression. The bacterial superantigens TSST-1, SEB and streptococcal pyrogenic exotoxin A (SPEA) were also unable to upregulate LT-beta mRNA transcript, in contrast to the observation with LT-alpha.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1043-4666
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
940-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10049517-Aging, pubmed-meshheading:10049517-Animals, pubmed-meshheading:10049517-Base Sequence, pubmed-meshheading:10049517-Concanavalin A, pubmed-meshheading:10049517-Cytokines, pubmed-meshheading:10049517-DNA Primers, pubmed-meshheading:10049517-Inflammation Mediators, pubmed-meshheading:10049517-Lipopolysaccharides, pubmed-meshheading:10049517-Lymphotoxin-alpha, pubmed-meshheading:10049517-Lymphotoxin-beta, pubmed-meshheading:10049517-Membrane Proteins, pubmed-meshheading:10049517-Mice, pubmed-meshheading:10049517-Mice, Inbred BALB C, pubmed-meshheading:10049517-RNA, Messenger, pubmed-meshheading:10049517-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10049517-Sepsis, pubmed-meshheading:10049517-Spleen, pubmed-meshheading:10049517-Superantigens, pubmed-meshheading:10049517-Transcription, Genetic, pubmed-meshheading:10049517-Up-Regulation
pubmed:year
1998
pubmed:articleTitle
The role of lipopolysaccharide, pro-inflammatory cytokines and bacterial superantigens in the transcriptional regulation of lymphotoxin alpha and beta in mouse splenocytes.
pubmed:affiliation
Department of Infectious Diseases, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't