Source:http://linkedlifedata.com/resource/pubmed/id/10037183
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-4-29
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pubmed:abstractText |
The mRNA expression of the tumor-associated antigens MAGE and GAGE was examined in 60 high-grade brain tumors. This analysis was performed by using reverse transcription-PCR, Southern blotting, and sequencing. It was demonstrated that, of the eight GAGE genes, GAGE-2 and -7 were expressed in five of seven normal brains. Four groups of tumors--adult glioblastoma multiforme (n = 20), pediatric glioblastoma multiforme (n = 9), medulloblastomas (n = 15), and ependymomas (n = 14)--were analyzed for mRNA expression. The following frequencies were observed: MAGE-1, 0, 0, 13, and 0%, respectively; MAGE-2, 5, 11, 60, and 57%; MAGE-3 & -6, 0, 0, 13, and 0%; GAGE-1, 65, 11, 13, and 43%; and GAGE-3-6 and -8: 75, 78, 47, and 93%, respectively. Two unclassified tumors expressed GAGE-3-6 and -8 only. The absence of GAGE-1 expression in normal brain, its relatively high frequency of expression in high-grade brain tumors, and its unique 3' sequence, suggest it may represent a useful target for specific immunotherapy. The detection method of reverse transcription-PCR and Southern blotting may also be useful for rapid screening of biopsy specimens both for diagnostic purposes and to determine a patient's eligibility for specific immunotherapy.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/GAGE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GAGE2A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MAGEA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MAGEB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Melanoma-Specific Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1078-0432
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-41
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10037183-Adult,
pubmed-meshheading:10037183-Antigens, Neoplasm,
pubmed-meshheading:10037183-Brain,
pubmed-meshheading:10037183-Brain Neoplasms,
pubmed-meshheading:10037183-Child,
pubmed-meshheading:10037183-Child, Preschool,
pubmed-meshheading:10037183-DNA, Neoplasm,
pubmed-meshheading:10037183-Fetus,
pubmed-meshheading:10037183-Humans,
pubmed-meshheading:10037183-Immunotherapy,
pubmed-meshheading:10037183-Melanoma-Specific Antigens,
pubmed-meshheading:10037183-Neoplasm Proteins,
pubmed-meshheading:10037183-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10037183-Tumor Cells, Cultured,
pubmed-meshheading:10037183-Tumor Markers, Biological
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pubmed:year |
1999
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pubmed:articleTitle |
Expression of MAGE and GAGE in high-grade brain tumors: a potential target for specific immunotherapy and diagnostic markers.
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pubmed:affiliation |
Department of Haematology and Oncology, Royal Children's Hospital, Melbourne, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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