|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1999-3-11
|
|
pubmed:abstractText |
We investigate here the effects of the incorporation of the nucleoside analogs araC (1-beta-D-arabinofuranosylcytosine) and ganciclovir (9-[(1,3-dihydroxy-2-propoxy)methyl] guanine) into the DNA binding recognition sequence for the GATA-1 erythroid transcription factor. A 10-fold decrease in binding affinity was observed for the ganciclovir-substituted DNA complex in comparison to an unmodified DNA of the same sequence composition. AraC substitution did not result in any changes in binding affinity. 1H-15N HSQC and NOESY NMR experiments revealed a number of chemical shift changes in both DNA and protein in the ganciclovir-modified DNA-protein complex when compared to the unmodified DNA-protein complex. These changes in chemical shift and binding affinity suggest a change in the binding mode of the complex when ganciclovir is incorporated into the GATA DNA binding site.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
444
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
47-53
|
|
pubmed:dateRevised |
2005-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:10037146-Animals,
pubmed-meshheading:10037146-Antimetabolites,
pubmed-meshheading:10037146-Base Sequence,
pubmed-meshheading:10037146-Binding Sites,
pubmed-meshheading:10037146-Chickens,
pubmed-meshheading:10037146-Cytarabine,
pubmed-meshheading:10037146-DNA,
pubmed-meshheading:10037146-DNA-Binding Proteins,
pubmed-meshheading:10037146-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:10037146-Ganciclovir,
pubmed-meshheading:10037146-Magnetic Resonance Spectroscopy,
pubmed-meshheading:10037146-Models, Molecular,
pubmed-meshheading:10037146-Nitrogen,
pubmed-meshheading:10037146-Nucleosides,
pubmed-meshheading:10037146-Protein Conformation,
pubmed-meshheading:10037146-Protons,
pubmed-meshheading:10037146-Response Elements,
pubmed-meshheading:10037146-Transcription Factors
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Effects of nucleoside analog incorporation on DNA binding to the DNA binding domain of the GATA-1 erythroid transcription factor.
|
|
pubmed:affiliation |
Walt Disney Memorial Cancer Institute at Florida Hospital, Orlando 32826, USA.
|
|
pubmed:publicationType |
Journal Article
|
