Linked Life Data

10027961

Source:http://linkedlifedata.com/resource/pubmed/id/10027961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-4-29
pubmed:abstractText
A new group of potent antibacterial compounds, non-iron metalloporphyrins (MPs), is described. MPs possess a strong antibacterial activity against Gram-positive bacteria, Gram-negative bacteria and mycobacteria. Anaerobically grown bacteria and microorganisms that do not respire and/or express haem uptake systems were resistant to MPs. Antibacterial activity of MPs was not affected by known antibiotic resistance mechanisms operating in bacteria. The most potent MP against Y. enterocolitica, methicillin-resistant S. aureus and M. smegmatis was gallium protoporphyrin IX (Ga-PPIX). When tested alone, Ga ions and metal-free porphyrins had approximately 100-fold higher minimum inhibitory concentration (MIC) values for these organisms. Ga-PPIX was not degraded by MP-sensitive bacteria, indicating that the whole molecule is responsible for antibacterial activity. MPs are antibacterial 'Trojan horses', as they exploit haem transport systems of Gram-negative bacteria as portals of entry into the cell. Bacterial mutants in superoxide dismutases, catalases and stationary-phase sigma factors were hypersensitive to Ga-PPIX. The extreme sensitivity of sod mutants to MPs and the requirement for active respiration for MP activity suggests that these compounds stimulate the production of reactive oxygen radicals in bacteria. Ga-PPIX was not toxic to primary human fibroblasts, several established cell lines and experimental animals at concentrations > 100-fold higher than the MIC for sensitive bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
429-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10027961-Anaerobiosis, pubmed-meshheading:10027961-Animals, pubmed-meshheading:10027961-Anti-Bacterial Agents, pubmed-meshheading:10027961-Caco-2 Cells, pubmed-meshheading:10027961-Cells, Cultured, pubmed-meshheading:10027961-Cercopithecus aethiops, pubmed-meshheading:10027961-Cytochromes, pubmed-meshheading:10027961-Dogs, pubmed-meshheading:10027961-Drug Resistance, Microbial, pubmed-meshheading:10027961-Escherichia coli, pubmed-meshheading:10027961-Female, pubmed-meshheading:10027961-Gallium, pubmed-meshheading:10027961-Gram-Negative Bacteria, pubmed-meshheading:10027961-Hemoglobins, pubmed-meshheading:10027961-Humans, pubmed-meshheading:10027961-Hydrogen Peroxide, pubmed-meshheading:10027961-Ions, pubmed-meshheading:10027961-Iron, pubmed-meshheading:10027961-Metalloporphyrins, pubmed-meshheading:10027961-Metals, pubmed-meshheading:10027961-Mice, pubmed-meshheading:10027961-Mice, Inbred BALB C, pubmed-meshheading:10027961-Microbial Sensitivity Tests, pubmed-meshheading:10027961-Mycobacterium smegmatis, pubmed-meshheading:10027961-Protoporphyrins, pubmed-meshheading:10027961-Staphylococcus aureus, pubmed-meshheading:10027961-Vero Cells, pubmed-meshheading:10027961-Yersinia enterocolitica
pubmed:year
1999
pubmed:articleTitle
Non-iron metalloporphyrins: potent antibacterial compounds that exploit haem/Hb uptake systems of pathogenic bacteria.
pubmed:affiliation
Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA. stojiljk@microbio1.microbio.emory.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't