Linked Life Data

10027879

Source:http://linkedlifedata.com/resource/pubmed/id/10027879

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-4-1
pubmed:abstractText
The purpose of the present study was to confirm the presence of beta3-adrenoceptor subtype in the relaxation of human urinary bladder detrusor tissue by reverse transcription-polymerase chain reaction (PCR); direct sequencing of the PCR product, in situ hybridization; and isometric contraction. Using reverse transcription-PCR, the mRNAs of three receptor subtypes (beta1, beta2, and beta3) were expressed in the human urinary bladder detrusor tissue. Direct sequencing of the PCR product of the above beta3-adrenoceptor revealed no mutation in the amplified regions. In situ hybridization with digoxygenin-labeled oligonucleotide probe revealed the presence of the mRNA of beta3-adrenoceptor subtype in the smooth muscle of the urinary bladder. The relaxant effects of isoproterenol (a nonselective beta-adrenoceptor agonist); ZD7114, BRL37344, and CGP12177A (putative selective beta3-adrenoceptor agonists); and SR59230A (a putative selective beta3-adrenoceptor antagonist) were tested using an isometric contraction technique. Isoproterenol in either the presence or absence of both atenolol (a beta1-adrenoceptor-selective antagonist) and butoxamine (a beta2-adrenoceptor-selective antagonist) revealed a relaxant effect on the carbachol-induced contraction of the human urinary bladder detrusor. Both BRL37344 and CGP12177A also revealed relaxant effects on the human urinary bladder detrusor, but ZD7114 did not elicit any relaxation. These results suggest that beta3-adrenoceptor may have some role in urine storage in the human urinary bladder.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1367-73
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10027879-Adrenergic beta-Agonists, pubmed-meshheading:10027879-Adrenergic beta-Antagonists, pubmed-meshheading:10027879-Aged, pubmed-meshheading:10027879-Base Sequence, pubmed-meshheading:10027879-Humans, pubmed-meshheading:10027879-In Situ Hybridization, pubmed-meshheading:10027879-Isometric Contraction, pubmed-meshheading:10027879-Isoproterenol, pubmed-meshheading:10027879-Middle Aged, pubmed-meshheading:10027879-Molecular Sequence Data, pubmed-meshheading:10027879-Muscle, Smooth, pubmed-meshheading:10027879-Propanolamines, pubmed-meshheading:10027879-RNA, Messenger, pubmed-meshheading:10027879-RNA Probes, pubmed-meshheading:10027879-Receptors, Adrenergic, beta, pubmed-meshheading:10027879-Receptors, Adrenergic, beta-3, pubmed-meshheading:10027879-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10027879-Urinary Bladder
pubmed:year
1999
pubmed:articleTitle
Evidence for beta3-adrenoceptor subtypes in relaxation of the human urinary bladder detrusor: analysis by molecular biological and pharmacological methods.
pubmed:affiliation
Department of Urology, Niigata University School of Medicine, Niigata, Japan. takedam@med.niigata-u.ac.jp
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't