Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-2-25
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058191, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058192, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058193, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058194, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058195, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058196, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058197, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058198, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058199, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058200, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058201, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058202, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058203, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058204, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058205, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058206, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058207, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058208, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058209, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058210, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058211, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058212, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058213, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058214, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058215, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058216, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058217, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058218, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058219, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AI058220
pubmed:abstractText
The aim of this study was to characterize the mRNA content of mammalian cochlear outer hair cells (OHCs) and to search for specific genes possibly involved in their unique properties. Indeed, OHCs, which feature high-frequency electromotility, are responsible for the exquisite sensitivity and frequency selectivity of the cochlea. Damage to these cells, which occurs in various conditions, causes a reduction in the cochlear sensitivity by about 50 dB and the alteration of frequency discrimination. Total RNA was extracted from about 2000 mechanically dissociated OHCs, and a polymerase chain reaction (PCR) amplified cDNA library was constructed. The presence of the alpha-9 acetylcholine receptor subunit, preferentially expressed in OHCs, was found by direct PCR amplification of the library. A systematic sequencing of 218 clones showed 78% known genes, 11% EST-related sequences, and 11% unknown genes. The known-gene group was characterized by two main features: a large proportion (55%) of mitochondrial transcripts and an abundance in calcium-binding proteins, such as calmodulin and calbindin, for which expression has already been demonstrated in OHCs. Another protein, the oncomodulin recently shown to be OHC specific, was also found, and its mRNA expression was confirmed by in situ hybridization. Among the 24 unknown genes, 7 were expressed in a restricted pattern, including one expressed in cochlea and spleen and, to a lesser extent, in lungs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10025504-Animals, pubmed-meshheading:10025504-Calcium-Binding Proteins, pubmed-meshheading:10025504-Cloning, Molecular, pubmed-meshheading:10025504-Cochlea, pubmed-meshheading:10025504-DNA, Complementary, pubmed-meshheading:10025504-DNA, Mitochondrial, pubmed-meshheading:10025504-Expressed Sequence Tags, pubmed-meshheading:10025504-Gene Expression, pubmed-meshheading:10025504-Gene Library, pubmed-meshheading:10025504-Hair Cells, Auditory, Outer, pubmed-meshheading:10025504-In Situ Hybridization, pubmed-meshheading:10025504-Lung, pubmed-meshheading:10025504-Organ Specificity, pubmed-meshheading:10025504-RNA, Messenger, pubmed-meshheading:10025504-Rats, pubmed-meshheading:10025504-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10025504-Sequence Analysis, DNA, pubmed-meshheading:10025504-Sequence Homology, Nucleic Acid, pubmed-meshheading:10025504-Spleen
pubmed:year
1999
pubmed:articleTitle
Expression pattern of mammalian cochlea outer hair cell (OHC) mRNA: screening of a rat OHC cDNA library.
pubmed:affiliation
INSERM U. 254 et Université Montpellier I, Hôpital Saint Charles, France.
pubmed:publicationType
Journal Article