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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-3-12
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pubmed:abstractText |
A hybrid protein [Met-Ala-(His)6OprF190-342-OprI21-83] consisting of the mature outer membrane protein I (OprI) and amino acids 190 to 342 of OprF of Pseudomonas aeruginosa was expressed in Escherichia coli and purified by Ni2+ chelate-affinity chromatography. After safety and pyrogenicity evaluations in animals, four groups of eight adult human volunteers were vaccinated intramuscularly three times at 4-week intervals and revaccinated 6 months later with either 500, 100, 50, or 20 microg of OprF-OprI adsorbed onto A1(OH)3. All vaccinations were well tolerated. After the first vaccination, a significant rise of antibody titers against P. aeruginosa OprF and OprI was measured in volunteers receiving the 100- or the 500-microg dose. After the second vaccination, significant antibody titers were measured for all groups. Elevated antibody titers against OprF and OprI could still be measured 6 months after the third vaccination. The capacity of the elicited antibodies to promote complement binding and opsonization could be demonstrated by a C1q-binding assay and by the in vitro opsonophagocytic uptake of P. aeruginosa bacteria. These data support the continued development of an OprF-OprI vaccine for use in humans.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-1438580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-1706316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-1940392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-1984637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2114360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2210853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2447060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2449095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2502533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2511252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2602775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-2833440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3033095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3102446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3110215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3121520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3131471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-3924612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-402473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-6183370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-6323316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-6799548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-7539410,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10024596-9673254
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1461-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10024596-Adult,
pubmed-meshheading:10024596-Antibodies, Bacterial,
pubmed-meshheading:10024596-Bacterial Proteins,
pubmed-meshheading:10024596-Bacterial Vaccines,
pubmed-meshheading:10024596-Female,
pubmed-meshheading:10024596-Humans,
pubmed-meshheading:10024596-Lipoproteins,
pubmed-meshheading:10024596-Male,
pubmed-meshheading:10024596-Phagocytosis,
pubmed-meshheading:10024596-Porins,
pubmed-meshheading:10024596-Pseudomonas aeruginosa,
pubmed-meshheading:10024596-Recombinant Fusion Proteins,
pubmed-meshheading:10024596-Vaccination,
pubmed-meshheading:10024596-Vaccines, Synthetic
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pubmed:year |
1999
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pubmed:articleTitle |
Safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers.
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pubmed:affiliation |
Chirurgische Universitätsklinik der Universität Freiburg, Freiburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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