Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-7-21
pubmed:abstractText
We engineered recombinant derivatives of simian immunodeficiency virus (SIV) to express enhanced green fluorescent protein (EGFP). Replacement of vpr sequences with EGFP resulted in a genome that did not produce detectable levels of replication-competent virus. Replication-competent virus and bright fluorescence of infected cells were obtained with two other constructs, one in which SIV nef sequences were replaced by EGFP and another in which EGFP was inserted into the SIV nef locus and HIV-1 nef sequences were expressed by downstream placement of an internal ribosomal entry site. These strains were infectious in rhesus monkeys and green fluorescing cells were detected in the tissues of infected monkeys by FACS analysis and by direct microscopic visualization. EGFP sequences were absent from recovered virus by 8 weeks following infection. We conclude that recombinant SIV that is engineered to express EGFP can be used to directly detect productively infected cells and aid in the immunophenotypic characterization of these cells within the first 2 weeks of infection of rhesus monkeys.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0889-2229
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10024048-Animals, pubmed-meshheading:10024048-Base Sequence, pubmed-meshheading:10024048-Cell Line, pubmed-meshheading:10024048-Flow Cytometry, pubmed-meshheading:10024048-Green Fluorescent Proteins, pubmed-meshheading:10024048-Immunohistochemistry, pubmed-meshheading:10024048-In Situ Hybridization, pubmed-meshheading:10024048-Indicators and Reagents, pubmed-meshheading:10024048-Luminescent Proteins, pubmed-meshheading:10024048-Macaca mulatta, pubmed-meshheading:10024048-Microscopy, Fluorescence, pubmed-meshheading:10024048-Molecular Sequence Data, pubmed-meshheading:10024048-Polymerase Chain Reaction, pubmed-meshheading:10024048-Recombinant Fusion Proteins, pubmed-meshheading:10024048-Simian Acquired Immunodeficiency Syndrome, pubmed-meshheading:10024048-Simian immunodeficiency virus, pubmed-meshheading:10024048-Transfection, pubmed-meshheading:10024048-Viral Load, pubmed-meshheading:10024048-Virus Replication
pubmed:year
1999
pubmed:articleTitle
Recombinant simian immunodeficiency virus expressing green fluorescent protein identifies infected cells in rhesus monkeys.
pubmed:affiliation
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't