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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-3-3
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pubmed:abstractText |
The two genetically established antimicrobial mechanisms of macrophages are production of reactive oxygen intermediates by phagocyte oxidase (phox) and reactive nitrogen intermediates by inducible nitric oxide synthase (NOS2). Mice doubly deficient in both enzymes (gp91(phox-/-)/NOS2(-/-)) formed massive abscesses containing commensal organisms, mostly enteric bacteria, even when reared under specific pathogen-free conditions with antibiotics. Neither parental strain showed such infections. Thus, phox and NOS2 appear to compensate for each other's deficiency in providing resistance to indigenous bacteria, and no other pathway does so fully. Macrophages from gp91(phox-/-)/NOS2(-/-) mice could not kill virulent Listeria. Their killing of S. typhimurium, E. coli, and attenuated Listeria was markedly diminished but demonstrable, establishing the existence of a mechanism of macrophage antibacterial activity independent of phox and NOS2.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-38
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10023768-Abscess,
pubmed-meshheading:10023768-Animals,
pubmed-meshheading:10023768-Bacterial Infections,
pubmed-meshheading:10023768-Crosses, Genetic,
pubmed-meshheading:10023768-Escherichia coli,
pubmed-meshheading:10023768-Genetic Predisposition to Disease,
pubmed-meshheading:10023768-Listeria monocytogenes,
pubmed-meshheading:10023768-Listeriosis,
pubmed-meshheading:10023768-Macrophages, Peritoneal,
pubmed-meshheading:10023768-Membrane Glycoproteins,
pubmed-meshheading:10023768-Mice,
pubmed-meshheading:10023768-Mice, Inbred C57BL,
pubmed-meshheading:10023768-Mice, Inbred Strains,
pubmed-meshheading:10023768-Mice, Knockout,
pubmed-meshheading:10023768-NADPH Oxidase,
pubmed-meshheading:10023768-Nitric Oxide Synthase,
pubmed-meshheading:10023768-Nitric Oxide Synthase Type II,
pubmed-meshheading:10023768-Phenotype,
pubmed-meshheading:10023768-Salmonella Infections, Animal,
pubmed-meshheading:10023768-Salmonella typhimurium
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pubmed:year |
1999
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pubmed:articleTitle |
Phenotype of mice and macrophages deficient in both phagocyte oxidase and inducible nitric oxide synthase.
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pubmed:affiliation |
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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