| pubmed-article:10023439 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C1564874 | lld:lifeskim |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:10023439 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:10023439 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10023439 | pubmed:dateCreated | 1999-3-9 | lld:pubmed |
| pubmed-article:10023439 | pubmed:abstractText | Recombinant adeno-associated virus (rAAV) vectors hold promise for treating a number of neurological disorders due to the ability to deliver long-term gene expression without toxicity or immune response. Critical to these endeavors will be controlled expression of the therapeutic gene in target cells. We have constructed and tested a dual cassette rAAV vector carrying a reporter gene under the control of the tetracycline-responsive system and the tetracycline transactivator. Transduction in vitro resulted in stable expression from the vector that can be suppressed 20-fold by tetracycline treatment. In vivo experiments, carried out to 6 weeks, demonstrated that vector-transduced expression is sustained until doxycycline administration upon which reporter gene expression is reduced. Moreover, the suppression of vector-driven expression can be reversed by removal of the drug. These studies demonstrate long-term regulated gene expression from rAAV vectors. This system will provide a valuable approach for controlling vector gene expression both in vitro and in vivo. | lld:pubmed |
| pubmed-article:10023439 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10023439 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10023439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10023439 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10023439 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10023439 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:10023439 | pubmed:author | pubmed-author:SamulskiR JRJ | lld:pubmed |
| pubmed-article:10023439 | pubmed:author | pubmed-author:McCownT JTJ | lld:pubmed |
| pubmed-article:10023439 | pubmed:author | pubmed-author:HabermanR PRP | lld:pubmed |
| pubmed-article:10023439 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10023439 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:10023439 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10023439 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10023439 | pubmed:pagination | 1604-11 | lld:pubmed |
| pubmed-article:10023439 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:10023439 | pubmed:meshHeading | pubmed-meshheading:10023439... | lld:pubmed |
| pubmed-article:10023439 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:10023439 | pubmed:articleTitle | Inducible long-term gene expression in brain with adeno-associated virus gene transfer. | lld:pubmed |
| pubmed-article:10023439 | pubmed:affiliation | UNC Gene Therapy Center, University of North Carolina, Chapel Hill 27599, USA. | lld:pubmed |
| pubmed-article:10023439 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10023439 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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