Source:http://linkedlifedata.com/resource/pubmed/id/10023439
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1999-3-9
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| pubmed:abstractText |
Recombinant adeno-associated virus (rAAV) vectors hold promise for treating a number of neurological disorders due to the ability to deliver long-term gene expression without toxicity or immune response. Critical to these endeavors will be controlled expression of the therapeutic gene in target cells. We have constructed and tested a dual cassette rAAV vector carrying a reporter gene under the control of the tetracycline-responsive system and the tetracycline transactivator. Transduction in vitro resulted in stable expression from the vector that can be suppressed 20-fold by tetracycline treatment. In vivo experiments, carried out to 6 weeks, demonstrated that vector-transduced expression is sustained until doxycycline administration upon which reporter gene expression is reduced. Moreover, the suppression of vector-driven expression can be reversed by removal of the drug. These studies demonstrate long-term regulated gene expression from rAAV vectors. This system will provide a valuable approach for controlling vector gene expression both in vitro and in vivo.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0969-7128
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1604-11
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10023439-Animals,
pubmed-meshheading:10023439-Brain,
pubmed-meshheading:10023439-Dependovirus,
pubmed-meshheading:10023439-Doxycycline,
pubmed-meshheading:10023439-Gene Expression,
pubmed-meshheading:10023439-Gene Therapy,
pubmed-meshheading:10023439-Genetic Vectors,
pubmed-meshheading:10023439-HeLa Cells,
pubmed-meshheading:10023439-Humans,
pubmed-meshheading:10023439-Male,
pubmed-meshheading:10023439-Microinjections,
pubmed-meshheading:10023439-Microscopy, Fluorescence,
pubmed-meshheading:10023439-Protein Synthesis Inhibitors,
pubmed-meshheading:10023439-Rats,
pubmed-meshheading:10023439-Rats, Sprague-Dawley,
pubmed-meshheading:10023439-Tetracycline,
pubmed-meshheading:10023439-Time Factors,
pubmed-meshheading:10023439-Transfection,
pubmed-meshheading:10023439-Transgenes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Inducible long-term gene expression in brain with adeno-associated virus gene transfer.
|
| pubmed:affiliation |
UNC Gene Therapy Center, University of North Carolina, Chapel Hill 27599, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|