CPATH-543905

Source:http://cbio.mskcc.org/cpath#CPATH-543905

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Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	http://www.biopax.org/release/biopax-level2.owl#catalysis
http://www.biopax.org/relea...	cpath:CPATH-LOCAL-7761873, cpath:CPATH-LOCAL-7765887
http://www.biopax.org/relea...	Ub-BTRC-IKBA
http://www.biopax.org/relea...	cpath:CPATH-543732
http://www.biopax.org/relea...	cpath:CPATH-LOCAL-7761870
http://www.biopax.org/relea...	PHYSIOL-LEFT-TO-RIGHT
http://www.biopax.org/relea...	cpath:CPATH-LOCAL-7761874
http://www.biopax.org/relea...	Ubiquitination of IKBA by BTRC.1) To assay IB ubiquitination activity in reconstituted mammalian extracts, GST--TRCP or GST proteins purified from insect cells; IkBalpha/p50/p65 complexes were also purified from insect cells.2) Crude extracts from THP.1 (human monocyte) cells was used for the assay.3) BTRC specifically recognizes a 19-amino-acid destruction motif in IkB-alpha (residues 21-41) in a phosphorylation-dependent manner for ubiquitination.