While the enzyme from Escherichia coli is highly specific for the 10-carbon enoyl-ACP, the enzyme from Streptococcus pneumoniae can also use the 12-carbon enoyl-ACP as substrate in vitro but not 14-or 16-carbon enoyl-ACPs.
Source:http://purl.uniprot.org/enzyme/5.3.3.14
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