The gene encoding enolase (eno) from the ammonia oxidising bacterium Nitrosomonas europaea has been cloned and sequenced. The deduced amino acid sequence for enolase from N. europaea was 65% identical (76% similar) to its Bacillus subtilis orthologue. An incomplete open reading frame located 432 bp 5' of eno was identified as pyrG, which encodes CTP synthase. These two genes are therefore organised in N. europaea, a beta-subdivision proteobacterium, in the same way as in the gamma-subdivision proteobacterium Escherichia coli.