http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
http://purl.uniprot.org/cit... | rdfs:comment | The entire cDNA sequences of a novel snake venom platelet glycoprotein (GP) Ib-binding protein (BP) composed of an alpha/beta heterodimeric structure, termed mamushigin, from Agkistrodon halys blomhoffii were determined, that include the leader peptides (21/23 amino acid residues) and mature subunits (136/123 amino acid residues). The mature subunits of mamushigin are 37.5% identical, and showed a high degree of similarity (37.7-67.5% identity) with the respective subunits of group VII C-type lectins (19). The sequences of the leader peptides of the mamusigin subunits showed the highest similarity (alpha-73.9/beta-82.6%) with those of factor IX/X-BP from Trimeresurus flavoviridis, and the cleavage site residue in both proteins was the same Ala(-1). The GPIb-binding specificity of mamushigin is strongly supported by several lines of evidence, but mamushigin can directly aggregate normal platelets, similar to alboaggregin-B (AL-B) (1). This differs from other GPIb-BP's. In mamushigin-treated platelets, serotonin was not released, and flow cytometric analysis using a monoclonal antibody PAC-1 totally excluded platelet GPIIb/IIIa activation. Mamushigin enhanced platelet aggregation at low-shear stress, and this effect totally disappeared in the presence of GPIb-receptor blockers specific for von Willebrand factor binding, but not by GPIIb/IIIa-receptor blockers. At high-shear stress, mamushigin blocked platelet aggregation in a dose-dependent manner, as seen with other GPIb-BP's. This paper, therefore, describes the cDNA cloning and molecular characterization of mamushigin which has a different effect on platelet aggregation under different shear stress. | lld:uniprot |
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http://purl.uniprot.org/cit... | uniprot:name | Thromb. Haemost. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Titani K. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Imamura K. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Suzuki M. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Matsui T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Kawasaki T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Kokubo T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Sakurai Y. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Yoshioka A. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Fujimura Y. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Handa M. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 1998 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 1199-1207 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | The cDNA cloning and molecular characterization of a snake venom platelet glycoprotein Ib-binding protein, mamushigin, from Agkistrodon halys blomhoffii venom. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 79 | lld:uniprot |
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