Curr. Microbiol.

The root-associated bacterium Azospirillum brasilense Sp7 produces the growth-stimulating phytohormone indole-3-acetic acid (= IAA) via the indole-3-pyruvate pathway. The DNA region containing ipdC, the structural gene for indole-3-pyruvate decarboxylase, was identified in a cosmid gene library of strain Sp7 by hybridization and has been sequenced. Upstream of the gene, two other ORF homologous to gltX and cysS were sequenced that are transcribed in the opposite direction. A functional analysis of the cloned ipdC region has been performed. To test the expression of the gene, a lacZ-Km cartridge was introduced into the gene. By this construct, tryptophan-dependent stimulation of gene expression in A. brasilense Sp7 was observed. Evidences for the existence of another copy of the ipdC gene in the Azospirillum genome are also reported.

Source:http://purl.uniprot.org/citations/9608743

Statements in which the resource exists.
SubjectPredicateObjectContext
http://purl.uniprot.org/cit...rdf:typeuniprot:Journal_Citationlld:uniprot
http://purl.uniprot.org/cit...rdfs:commentThe root-associated bacterium Azospirillum brasilense Sp7 produces the growth-stimulating phytohormone indole-3-acetic acid (= IAA) via the indole-3-pyruvate pathway. The DNA region containing ipdC, the structural gene for indole-3-pyruvate decarboxylase, was identified in a cosmid gene library of strain Sp7 by hybridization and has been sequenced. Upstream of the gene, two other ORF homologous to gltX and cysS were sequenced that are transcribed in the opposite direction. A functional analysis of the cloned ipdC region has been performed. To test the expression of the gene, a lacZ-Km cartridge was introduced into the gene. By this construct, tryptophan-dependent stimulation of gene expression in A. brasilense Sp7 was observed. Evidences for the existence of another copy of the ipdC gene in the Azospirillum genome are also reported.lld:uniprot
http://purl.uniprot.org/cit...skos:exactMatchhttp://purl.uniprot.org/med...lld:uniprot
http://purl.uniprot.org/cit...skos:exactMatchhttp://purl.uniprot.org/pub...lld:uniprot
http://purl.uniprot.org/cit...uniprot:nameCurr. Microbiol.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorZimmer W.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorWesche M.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorTimmermans L.lld:uniprot
http://purl.uniprot.org/cit...uniprot:date1998lld:uniprot
http://purl.uniprot.org/cit...uniprot:pages327-331lld:uniprot
http://purl.uniprot.org/cit...uniprot:titleIdentification and isolation of the indole-3-pyruvate decarboxylase gene from Azospirillum brasilense Sp7: sequencing and functional analysis of the gene locus.lld:uniprot
http://purl.uniprot.org/cit...uniprot:volume36lld:uniprot
http://purl.uniprot.org/cit...dc-term:identifierdoi:10.1007/s002849900317lld:uniprot
uniprot-protein:O07045uniprot:citationhttp://purl.uniprot.org/cit...lld:uniprot
uniprot-protein:P45631uniprot:citationhttp://purl.uniprot.org/cit...lld:uniprot
http://purl.uniprot.org/emb...uniprot:citationhttp://purl.uniprot.org/cit...lld:uniprot
http://linkedlifedata.com/r...rdf:objecthttp://purl.uniprot.org/cit...lld:uniprot
http://linkedlifedata.com/r...rdf:objecthttp://purl.uniprot.org/cit...lld:uniprot