The structure of R-phycoerythrin (R-PE) from Polysiphonia urceolata was determined at 2.8 A resolution. The crystals belong to space group R3 with unit cell dimensions of a = b = 189.8 A, c = 60.1 A. The subunit composition of R-PE is (alpha 2 beta 2)3 gamma. The three-dimensional structure of R-PE was solved by the multiple isomorphous replacement method. After several cycles of model building and refinement, the crystallographic R-factor of the final model is 18.0% with data from 10.0 to 2.8 A resolution. The four phycoerythrobilin chromophores alpha 84, alpha 140a, beta 84 and beta 155 in an (alpha beta) unit are each covalently bound to a cysteine residue through ring A. The phycourobilin chromophore is bound to cysteine beta 50 by ring A and bound to cysteine beta 61 by ring D. The ring A and ring D of phycourobilin deviate from the conjugate plane formed by ring B and ring C and the four rings form a boat-shaped structure. R-PE contains a 34 kDa gamma subunit that is assumed to lie in the central channel of the molecular disc (alpha 2 beta 2)3. The energy transfer and relationship between cysteine residues and chromophores are discussed.
Subject | Predicate | Object | Context |
---|---|---|---|
http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
http://purl.uniprot.org/cit... | rdfs:comment | The structure of R-phycoerythrin (R-PE) from Polysiphonia urceolata was determined at 2.8 A resolution. The crystals belong to space group R3 with unit cell dimensions of a = b = 189.8 A, c = 60.1 A. The subunit composition of R-PE is (alpha 2 beta 2)3 gamma. The three-dimensional structure of R-PE was solved by the multiple isomorphous replacement method. After several cycles of model building and refinement, the crystallographic R-factor of the final model is 18.0% with data from 10.0 to 2.8 A resolution. The four phycoerythrobilin chromophores alpha 84, alpha 140a, beta 84 and beta 155 in an (alpha beta) unit are each covalently bound to a cysteine residue through ring A. The phycourobilin chromophore is bound to cysteine beta 50 by ring A and bound to cysteine beta 61 by ring D. The ring A and ring D of phycourobilin deviate from the conjugate plane formed by ring B and ring C and the four rings form a boat-shaped structure. R-PE contains a 34 kDa gamma subunit that is assumed to lie in the central channel of the molecular disc (alpha 2 beta 2)3. The energy transfer and relationship between cysteine residues and chromophores are discussed. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | J. Mol. Biol. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Chang W.-R. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Jiang T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Wan Z.-L. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Zhang J.-P. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Yang Z.-X. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Liang D.-C. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 1996 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 721-731 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | Crystal structure of R-phycoerythrin from Polysiphonia urceolata at 2.8 A resolution. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 262 | lld:uniprot |
http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1006/jmbi.1996.0547 | lld:uniprot |
uniprot-protein:P84861 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
uniprot-protein:P84862 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |