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FEBS Lett.

We cloned a cDNA for the rat kappa-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by kappa-specific opioid ligands, but not by mu- and delta-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that kappa-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.

Source:http://purl.uniprot.org/citations/8103466

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http://purl.uniprot.org/cit...rdfs:commentWe cloned a cDNA for the rat kappa-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by kappa-specific opioid ligands, but not by mu- and delta-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that kappa-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.lld:uniprot
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http://purl.uniprot.org/cit...uniprot:authorNakamura S.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorKaneko S.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorMaekawa K.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorMinami M.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorSatoh M.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorToya T.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorKatao Y.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorOnogi T.lld:uniprot
http://purl.uniprot.org/cit...uniprot:date1993lld:uniprot
http://purl.uniprot.org/cit...uniprot:pages291-295lld:uniprot
http://purl.uniprot.org/cit...uniprot:titleCloning and expression of a cDNA for the rat kappa-opioid receptor.lld:uniprot
http://purl.uniprot.org/cit...uniprot:volume329lld:uniprot
http://purl.uniprot.org/cit...dc-term:identifierdoi:10.1016/0014-5793(93)80240-Ulld:uniprot
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