We report the genetic and biochemical properties of a staurosporine (ST)- and temperature-sensitive mutant, stt10, of Saccharomyces cerevisiae. The stt10 mutant shows an osmoremedial phenotype in a medium with 1 M sorbitol. ST sensitivity of the stt10 mutant was suppressed by overexpression of PKC1/STT1, showing the genetic interactions of STT10 with the PKC1/STT1 pathway. The nucleotide sequence of STT10 predicts a hydrophilic protein composed of 577 amino acids that possesses 20-25% sequence similarity with yeast Slp1/Vam5p, Sec1p and Sly1p, and nematode Unc-18. The stt10 deletion mutant is viable and shows a typical class-D vacuolar protein sorting defective (vps) phenotype. Vacuoles from stt10 cells have a normal vacuolar H(+)-ATPase activity, but are defective in vacuolar acidification. Genetic studies of yeast mutants carrying delta stt10, delta bck1, stt1/pkc1 or stt4 have revealed that their functions are phenotypically related to maintenance of cellular osmotic integrity.
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| http://purl.uniprot.org/cit... | rdfs:comment | We report the genetic and biochemical properties of a staurosporine (ST)- and temperature-sensitive mutant, stt10, of Saccharomyces cerevisiae. The stt10 mutant shows an osmoremedial phenotype in a medium with 1 M sorbitol. ST sensitivity of the stt10 mutant was suppressed by overexpression of PKC1/STT1, showing the genetic interactions of STT10 with the PKC1/STT1 pathway. The nucleotide sequence of STT10 predicts a hydrophilic protein composed of 577 amino acids that possesses 20-25% sequence similarity with yeast Slp1/Vam5p, Sec1p and Sly1p, and nematode Unc-18. The stt10 deletion mutant is viable and shows a typical class-D vacuolar protein sorting defective (vps) phenotype. Vacuoles from stt10 cells have a normal vacuolar H(+)-ATPase activity, but are defective in vacuolar acidification. Genetic studies of yeast mutants carrying delta stt10, delta bck1, stt1/pkc1 or stt4 have revealed that their functions are phenotypically related to maintenance of cellular osmotic integrity. | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/med... | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:name | Gene | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Anraku Y. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Yoshida S. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Ohya Y. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Nakano A. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Hirose R. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:date | 1995 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:pages | 117-122 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:title | STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:volume | 160 | lld:uniprot |
| http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1016/0378-1119(95)00214-Q | lld:uniprot |
| uniprot-protein:P38932 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
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