Two forms of storage proteins termed "SP 1" and "SP 2", respectively, accumulate in a stage-dependent fashion in the larval hemolymph of the silkworm Bombyx mori. We have cloned and analyzed the genomic sequence encoding SP 2, an arylphorin-type storage protein. The SP 2 structural gene contains five exons spanning over 5 kilobases of chromosomal DNA. The transcription initiation site of the SP 2 gene was identified at the nucleotide level and a typical TATA box was located 30 base pairs upstream of the transcription initiation site. The 5'-flanking region of the gene contains a sequence homologous to the SV 40 enhancer "core" structure. A region homologous to the Drosophila enhancer sequence predicted for the fat body specificity of gene expression exists in the 5'-upstream region. Developmental profiles of the SP 2 mRNA and its precursor in the fat body indicate that the biosynthesis of SP 2 is developmentally regulated at the level of transcription. The exon/intron composition of the SP 2 gene is remarkably similar to that of the SP 1 gene. Moreover, the deduced primary structures of SP 1 and SP 2 exhibit nearly 30% homology, implying that two storage protein genes of the B. mori silkworm might have evolved from a common ancestor.
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http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
http://purl.uniprot.org/cit... | rdfs:comment | Two forms of storage proteins termed "SP 1" and "SP 2", respectively, accumulate in a stage-dependent fashion in the larval hemolymph of the silkworm Bombyx mori. We have cloned and analyzed the genomic sequence encoding SP 2, an arylphorin-type storage protein. The SP 2 structural gene contains five exons spanning over 5 kilobases of chromosomal DNA. The transcription initiation site of the SP 2 gene was identified at the nucleotide level and a typical TATA box was located 30 base pairs upstream of the transcription initiation site. The 5'-flanking region of the gene contains a sequence homologous to the SV 40 enhancer "core" structure. A region homologous to the Drosophila enhancer sequence predicted for the fat body specificity of gene expression exists in the 5'-upstream region. Developmental profiles of the SP 2 mRNA and its precursor in the fat body indicate that the biosynthesis of SP 2 is developmentally regulated at the level of transcription. The exon/intron composition of the SP 2 gene is remarkably similar to that of the SP 1 gene. Moreover, the deduced primary structures of SP 1 and SP 2 exhibit nearly 30% homology, implying that two storage protein genes of the B. mori silkworm might have evolved from a common ancestor. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/med... | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | J. Biol. Chem. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Tomino S. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Fujii T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Izumi S. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Sakurai H. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 1989 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 11020-11025 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | Structure of the gene for the arylphorin-type storage protein SP 2 of Bombyx mori. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 264 | lld:uniprot |
uniprot-protein:P20613 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |