The human cytomegalovirus (HCMV) genes UL128, UL130 and UL131A are essential for endothelial cell infection. Complementation of the defective UL131A gene of the non-endotheliotropic HCMV strain AD169 with wild-type UL131A in cis in an ectopic position restored endothelial cell tropism. The UL131A protein was found in virions in a complex with gH. Coinfection of fibroblasts with UL131A-negative and -positive viruses restored the endothelial cell tropism of UL131A-negative virions by complementing the virions with UL131A protein. Virus entry into endothelial cells, but not into fibroblasts, was blocked by an antipeptide antiserum to pUL131A. AD169, cis-complemented with wild-type UL131A, showed an impaired release of infectious particles from fibroblasts. A comparable defect in virus release was observed when UL131A was expressed ectopically in a virus background already expressing an intact copy of UL131A. In contrast, virus release from infected endothelial cells was not affected by UL131A. These data suggest a dual role for pUL131A in virus entry and virus exit from infected cells.
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http://purl.uniprot.org/cit... | rdfs:comment | The human cytomegalovirus (HCMV) genes UL128, UL130 and UL131A are essential for endothelial cell infection. Complementation of the defective UL131A gene of the non-endotheliotropic HCMV strain AD169 with wild-type UL131A in cis in an ectopic position restored endothelial cell tropism. The UL131A protein was found in virions in a complex with gH. Coinfection of fibroblasts with UL131A-negative and -positive viruses restored the endothelial cell tropism of UL131A-negative virions by complementing the virions with UL131A protein. Virus entry into endothelial cells, but not into fibroblasts, was blocked by an antipeptide antiserum to pUL131A. AD169, cis-complemented with wild-type UL131A, showed an impaired release of infectious particles from fibroblasts. A comparable defect in virus release was observed when UL131A was expressed ectopically in a virus background already expressing an intact copy of UL131A. In contrast, virus release from infected endothelial cells was not affected by UL131A. These data suggest a dual role for pUL131A in virus entry and virus exit from infected cells. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | J. Gen. Virol. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Adler B. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Rupp B. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Sinzger C. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Koszinowski U. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Scrivano L. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Ruzcics Z. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 2006 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 2451-2460 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | Role of human cytomegalovirus UL131A in cell type-specific virus entry and release. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 87 | lld:uniprot |
http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1099/vir.0.81921-0 | lld:uniprot |
uniprot-protein:F5HET4 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |