http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
http://purl.uniprot.org/cit... | rdfs:comment | Tat is a critical viral transactivator essential for human immunodeficiency virus (HIV) gene expression. Activation involves binding to an RNA stem-loop structure and recruitment of the positive transcription elongation factor b. Tat also induces the remodeling of a single nucleosome in the HIV promoter. However, the mechanism of this remodeling has remained unclear. Knockdown of INI-1 and BRG-1, two components of the SWI/SNF chromatin-remodeling complex, suppressed Tat-mediated transactivation. Cells lacking INI-1 (G401 and MON) or BRG-1 (C33A) exhibited defective transactivation by Tat that was restored upon INI-1 and BRG-1 expression, respectively. Tat was co-immunoprecipitated with several SWI/SNF subunits, including INI-1, BRG-1, and beta-actin. The SWI/SNF complex interacted with the integrated HIV promoter in a Tat-dependent manner. We also found that INI-1 and BRG-1 synergized with the p300 acetyltransferase to activate the HIV promoter. This synergism depended on the acetyltransferase activity of p300 and on Tat Lys(50) and Lys(51). In conclusion, Tat-mediated activation of the HIV promoter requires the SWI/SNF complex in synergy with the coactivator p300. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | J. Biol. Chem. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Verrijzer C.P. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Ott M. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Verdin E. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Parra M. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Mahmoudi T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Vries R.G. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Kauder S.E. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 2006 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 19960-19968 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | The SWI/SNF chromatin-remodeling complex is a cofactor for Tat transactivation of the HIV promoter. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 281 | lld:uniprot |
http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1074/jbc.M603336200 | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |