A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
| Subject | Predicate | Object | Context |
|---|---|---|---|
| http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
| http://purl.uniprot.org/cit... | rdfs:comment | A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins. | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/med... | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:name | Plant Mol. Biol. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Albert H.A. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Martin T. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Sun S.S. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:date | 1992 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:pages | 663-671 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:title | Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:volume | 20 | lld:uniprot |
| http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1007/BF00046451 | lld:uniprot |
| uniprot-protein:P29193 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |