We describe the sequence and characterization of the Bacillus subtilis flhF gene. flhF encodes a basic polypeptide of 41 kDa that contains a putative GTP-binding motif. The sequence of FlhF reveals a structural relationship to two Escherichia coli proteins, Ffh and FtsY, as well as to other members of the SRP54 family, in a domain presumed to bind GTP. flhF is located in a large operon consisting of chemotaxis and flagellar genes. Cells deficient in flhF are nonmotile. Through the use of anti-flagellar antibodies we have established that flhF is a flagellar (fla) gene. Thus, flhF is a unique flagellar gene in that it encodes a GTP-binding protein with similarities to members of the SRP54 family of proteins. These data suggest that flagellar biosynthesis in B. subtilis requires GTP.
| Subject | Predicate | Object | Context |
|---|---|---|---|
| http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
| http://purl.uniprot.org/cit... | rdfs:comment | We describe the sequence and characterization of the Bacillus subtilis flhF gene. flhF encodes a basic polypeptide of 41 kDa that contains a putative GTP-binding motif. The sequence of FlhF reveals a structural relationship to two Escherichia coli proteins, Ffh and FtsY, as well as to other members of the SRP54 family, in a domain presumed to bind GTP. flhF is located in a large operon consisting of chemotaxis and flagellar genes. Cells deficient in flhF are nonmotile. Through the use of anti-flagellar antibodies we have established that flhF is a flagellar (fla) gene. Thus, flhF is a unique flagellar gene in that it encodes a GTP-binding protein with similarities to members of the SRP54 family of proteins. These data suggest that flagellar biosynthesis in B. subtilis requires GTP. | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/med... | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:name | Mol. Microbiol. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Carpenter P.B. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Hanlon D.W. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Ordal G.W. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:date | 1992 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:pages | 2705-2713 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:title | flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:volume | 6 | lld:uniprot |
| http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1111/j.1365-2958.1992.tb01447.x | lld:uniprot |
| uniprot-protein:Q01960 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |