A monoclonal antibody (2A5B9), previously shown to be reactive with a 14 kD surface associated antigen of Onchocerca microfilariae, was found to recognise a 92 kD molecule present in an adult worm extract. The antibody was used to select cDNA clones with a coding capacity larger than 14 kD, from a lambda gt11 library of O. volvulus. Nucleotide sequencing of the cDNA of one such clone revealed extensive homology to the myosin (unc-54) and paramyosin (unc-15) genes of Caenorhabditis elegans, similarly to myosin and paramyosin genes of Onchocerca volvulus, Brugia malayi, Dirofilaria immitis and Schistosoma mansoni. The immunological implications of antigenic cross-reactivity between a surface molecule and paramyosin, a known protective antigen, are discussed.
| Subject | Predicate | Object | Context |
|---|---|---|---|
| http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
| http://purl.uniprot.org/cit... | rdfs:comment | A monoclonal antibody (2A5B9), previously shown to be reactive with a 14 kD surface associated antigen of Onchocerca microfilariae, was found to recognise a 92 kD molecule present in an adult worm extract. The antibody was used to select cDNA clones with a coding capacity larger than 14 kD, from a lambda gt11 library of O. volvulus. Nucleotide sequencing of the cDNA of one such clone revealed extensive homology to the myosin (unc-54) and paramyosin (unc-15) genes of Caenorhabditis elegans, similarly to myosin and paramyosin genes of Onchocerca volvulus, Brugia malayi, Dirofilaria immitis and Schistosoma mansoni. The immunological implications of antigenic cross-reactivity between a surface molecule and paramyosin, a known protective antigen, are discussed. | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/med... | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:name | Trop. Med. Parasitol. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Conraths F.J. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Harnett H.W. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Worms M.J. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Parkhouse R.M. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:date | 1992 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:pages | 135-138 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:title | Immunological cross-reaction between an Onchocerca paramyosin-like molecule and a microfilaria surface antigen. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:volume | 43 | lld:uniprot |
| uniprot-protein:Q25630 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |