In this paper, we report the cDNA sequence of bovine thioredoxin. We determined the full-length cDNA sequence of bovine thioredoxin by RT-PCR, 5'-RACE and 3'-RACE methods. Currently, the thioredoxin cDNA sequences of only five mammalian species (human, macaca, mouse, ovine and rat) are registered in the GenBank database. We performed sequence comparisons on the total cDNA sequence and the coding region, and produced a multialignment between the amino acid sequences of bovine and other mammalian thioredoxins. The amino acid sequences of thioredoxins are highly conserved among mammalian species, for example, only one difference exists between the amino acid sequences of bovine and ovine thioredoxin.