http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
http://purl.uniprot.org/cit... | rdfs:comment | Ubiquinone (coenzyme Q or Q) is a lipid that functions in the electron transport chain in the inner mitochondrial membrane of eukaryotes and the plasma membrane of prokaryotes. Q-deficient mutants of Saccharomyces cerevisiae harbor defects in one of eight COQ genes (coq1-coq8) and are unable to grow on nonfermentable carbon sources. The biosynthesis of Q involves two separate O-methylation steps. In yeast, the first O-methylation utilizes 3, 4-dihydroxy-5-hexaprenylbenzoic acid as a substrate and is thought to be catalyzed by Coq3p, a 32.7-kDa protein that is 40% identical to the Escherichia coli O-methyltransferase, UbiG. In this study, farnesylated analogs corresponding to the second O-methylation step, demethyl-Q(3) and Q(3), have been chemically synthesized and used to study Q biosynthesis in yeast mitochondria in vitro. Both yeast and rat Coq3p recognize the demethyl-Q(3) precursor as a substrate. In addition, E. coli UbiGp was purified and found to catalyze both O-methylation steps. Futhermore, antibodies to yeast Coq3p were used to determine that the Coq3 polypeptide is peripherally associated with the matrix-side of the inner membrane of yeast mitochondria. The results indicate that one O-methyltransferase catalyzes both steps in Q biosynthesis in eukaryotes and prokaryotes and that Q biosynthesis is carried out within the matrix compartment of yeast mitochondria. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | J. Biol. Chem. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Clarke C.F. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Hsu A.Y. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Poon W.W. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Myles D.C. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Lee P.T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Barkovich R.J. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Frankel A. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Shepherd J.N. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 1999 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 21665-21672 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 274 | lld:uniprot |
http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1074/jbc.274.31.21665 | lld:uniprot |
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