| pubmed-article:9892654 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9892654 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:9892654 | lifeskim:mentions | umls-concept:C0013856 | lld:lifeskim |
| pubmed-article:9892654 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9892654 | pubmed:dateCreated | 1999-3-16 | lld:pubmed |
| pubmed-article:9892654 | pubmed:abstractText | Megabase DNA molecules become trapped in agarose gels during electrophoresis if the electric field exceeds a few volts per cm. Fluorescence microscopy reveals that these molecules invariably arrest in U-shaped conformations. The field-vs.-size dependence for trapping indicates that a critical molecular tension is required for trapping. The size of unligated lambda-ladders, sheared during gel electrophoresis at a given field, coincides with the size of molecules trapped at that field, suggesting that both processes occur through nick melting near the vertex of the U-shape. Consistently, molecules nicked by exposure to UV radiation trap more readily than unexposed ones. The critical trapping tension at the vertex is estimated to be 15 pN, a force sufficient to melt nicks bent around gel fibers, and, according to our model, trap a molecule. Strategies to reduce molecular tension and avoid trapping are discussed. | lld:pubmed |
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| pubmed-article:9892654 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9892654 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9892654 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9892654 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9892654 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:9892654 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9892654 | pubmed:author | pubmed-author:SmithS BSB | lld:pubmed |
| pubmed-article:9892654 | pubmed:author | pubmed-author:BustamanteCC | lld:pubmed |
| pubmed-article:9892654 | pubmed:author | pubmed-author:GurrieriSS | lld:pubmed |
| pubmed-article:9892654 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9892654 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:9892654 | pubmed:volume | 96 | lld:pubmed |
| pubmed-article:9892654 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9892654 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9892654 | pubmed:pagination | 453-8 | lld:pubmed |
| pubmed-article:9892654 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9892654 | pubmed:meshHeading | pubmed-meshheading:9892654-... | lld:pubmed |
| pubmed-article:9892654 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9892654 | pubmed:articleTitle | Trapping of megabase-sized DNA molecules during agarose gel electrophoresis. | lld:pubmed |
| pubmed-article:9892654 | pubmed:affiliation | Dipartimento di Scienze Chimiche, Universitá di Catania, Viale A. Doria 6, 95125 Catania, Italy. | lld:pubmed |
| pubmed-article:9892654 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9892654 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9892654 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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