| pubmed-article:9852157 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C0205378 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C1166758 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C0031621 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C1156237 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C0599896 | lld:lifeskim |
| pubmed-article:9852157 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9852157 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9852157 | pubmed:dateCreated | 1999-1-26 | lld:pubmed |
| pubmed-article:9852157 | pubmed:abstractText | We have examined the roles of the p85/ p110alpha and hVPS34 phosphatidylinositol (PI) 3'-kinases in cellular signaling using inhibitory isoform-specific antibodies. We raised anti-hVPS34 and anti-p110alpha antibodies that specifically inhibit recombinant hVPS34 and p110alpha, respectively, in vitro. We used the antibodies to study cellular processes that are sensitive to low-dose wortmannin. The antibodies had distinct effects on the actin cytoskeleton; microinjection of anti-p110alpha antibodies blocked insulin-stimulated ruffling, whereas anti-hVPS34 antibodies had no effect. The antibodies also had different effects on vesicular trafficking. Microinjection of inhibitory anti-hVPS34 antibodies, but not anti-p110alpha antibodies, blocked the transit of internalized PDGF receptors to a perinuclear compartment, and disrupted the localization of the early endosomal protein EEA1. Microinjection of anti-p110alpha antibodies, and to a lesser extent anti-hVPS34 antibodies, reduced the rate of transferrin recycling in CHO cells. Surprisingly, both antibodies inhibited insulin-stimulated DNA synthesis by 80%. Injection of cells with antisense oligonucleotides derived from the hVPS34 sequence also blocked insulin-stimulated DNA synthesis, whereas scrambled oligonucleotides had no effect. Interestingly, the requirement for p110alpha and hVPS34 occurred at different times during the G1-S transition. Our data suggest that different PI 3'-kinases play distinct regulatory roles in the cell, and document an unexpected role for hVPS34 during insulin-stimulated mitogenesis. | lld:pubmed |
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| pubmed-article:9852157 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9852157 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9852157 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9852157 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9852157 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9852157 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9852157 | pubmed:issn | 0021-9525 | lld:pubmed |
| pubmed-article:9852157 | pubmed:author | pubmed-author:ShahAA | lld:pubmed |
| pubmed-article:9852157 | pubmed:author | pubmed-author:BackerJ MJM | lld:pubmed |
| pubmed-article:9852157 | pubmed:author | pubmed-author:ZhangYY | lld:pubmed |
| pubmed-article:9852157 | pubmed:author | pubmed-author:McIlroyJJ | lld:pubmed |
| pubmed-article:9852157 | pubmed:author | pubmed-author:SiddhantaUU | lld:pubmed |
| pubmed-article:9852157 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9852157 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:9852157 | pubmed:volume | 143 | lld:pubmed |
