| pubmed-article:9728043 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9728043 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9728043 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:9728043 | lifeskim:mentions | umls-concept:C0458827 | lld:lifeskim |
| pubmed-article:9728043 | lifeskim:mentions | umls-concept:C0033554 | lld:lifeskim |
| pubmed-article:9728043 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:9728043 | pubmed:issue | 3 Pt 1 | lld:pubmed |
| pubmed-article:9728043 | pubmed:dateCreated | 1998-10-5 | lld:pubmed |
| pubmed-article:9728043 | pubmed:abstractText | We have previously reported that pretreatment of cultured human airway smooth muscle (HASM) cells with interleukin-1beta (IL-1beta) results in decreased beta-adrenergic responsiveness. The purpose of this study was to determine whether prostanoids released as a result of cyclooxygenase-2 (COX-2) induction by IL-1beta contribute to this effect of the cytokine. Confluent serum-deprived HASM cells were studied in passages 4-7. IL-1beta (20 ng/ml for 22 h) reduced the ability of the beta-agonist isoproterenol (Iso) to decrease stiffness of HASM cells as measured by magnetic twisting cytometry. The effect of IL-1beta on Iso-induced changes in cell stiffness was abolished by nonselective [indomethacin (Indo), 10(-6) M] and selective (NS-398, 10(-5) M) COX-2 inhibitors. Indo and NS-398 also inhibited both the increased basal cAMP and the decreases in Iso-stimulated cAMP production induced by IL-1beta. IL-1beta (20 ng/ml for 22 h) caused an increase in both basal (15-fold) and arachidonic acid (AA)-stimulated (10-fold) PGE2 release. Indo blocked basal and AA-stimulated PGE2 release in both control and IL-1beta-treated cells. NS-398 also markedly reduced basal and AA-stimulated PGE2 release in IL-1beta-treated cells but had no significant effect on AA-stimulated PGE2 release in control cells. Western blot analysis confirmed the induction of COX-2 by IL-1beta. Exogenously administered PGE2 (10(-7) M, 22 h) caused a significant reduction in the ability of Iso to decrease cell stiffness, mimicking the effects of IL-1beta. Cycloheximide (10 microg/ml for 24 h), an inhibitor of protein synthesis, also abolished the effects of IL-1beta on Iso-induced cell stiffness changes and cAMP formation. In summary, our results indicate that IL-1beta significantly increases prostanoid release by HASM cells as a result of increased COX-2 expression. The prostanoids appear to contribute to beta-adrenergic hyporesponsiveness, perhaps by heterologous desensitization of the beta2 receptor. | lld:pubmed |
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| pubmed-article:9728043 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:9728043 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9728043 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9728043 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9728043 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:MoellerWW | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:HeyderJJ | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:ShoreS ASA | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:PanettieriR... | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:MooreP EPE | lld:pubmed |
| pubmed-article:9728043 | pubmed:author | pubmed-author:LaporteJ DJD | lld:pubmed |
| pubmed-article:9728043 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9728043 | pubmed:volume | 275 | lld:pubmed |
| pubmed-article:9728043 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9728043 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9728043 | pubmed:pagination | L491-501 | lld:pubmed |
| pubmed-article:9728043 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9728043 | pubmed:meshHeading | pubmed-meshheading:9728043-... | lld:pubmed |
| pubmed-article:9728043 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9728043 | pubmed:articleTitle | Prostanoids mediate IL-1beta-induced beta-adrenergic hyporesponsiveness in human airway smooth muscle cells. | lld:pubmed |
| pubmed-article:9728043 | pubmed:affiliation | Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115, USA. | lld:pubmed |
| pubmed-article:9728043 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9728043 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9728043 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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