| pubmed-article:9654445 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9654445 | lifeskim:mentions | umls-concept:C1549081 | lld:lifeskim |
| pubmed-article:9654445 | lifeskim:mentions | umls-concept:C1692758 | lld:lifeskim |
| pubmed-article:9654445 | lifeskim:mentions | umls-concept:C1550009 | lld:lifeskim |
| pubmed-article:9654445 | lifeskim:mentions | umls-concept:C0052908 | lld:lifeskim |
| pubmed-article:9654445 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9654445 | pubmed:dateCreated | 1998-8-5 | lld:pubmed |
| pubmed-article:9654445 | pubmed:abstractText | Two types of sites are known to cause pausing and/or termination by bacteriophage T7 RNA polymerase (RNAP). Termination at class I sites (typified by the signal found in the late region of T7 DNA, TPhi) involves the formation of a stable stem-loop structure in the nascent RNA ahead of the point of termination, and results in termination near runs of U. Class II sites, typified by a signal first identified in the cloned human preproparathyroid hormone (PTH) gene, generate no evident structure in the RNA but contain a conserved sequence ahead of the point of termination, and also contain runs of U. Termination at class I and class II sites may involve non-equivalent mechanisms, as mutants of T7 RNA polymerase have been identified that fail to recognize class II sites yet continue to recognize class I sites. In this work, we have analyzed pausing and termination at several class II sites, and variants of them. We conclude that the 7 bp sequence ATCTGTT (5' to 3' in the non-template strand) causes transcribing T7 or T3 RNA polymerase to pause. Termination 6 to 8 bp past this sequence is favored by the presence of runs of U, perhaps because they destabilize an RNA:DNA hybrid. The effects of T7 lysozyme on pausing and termination are consistent with the idea that termination involves a reversion of the polymerase from the elongation to the initiation conformation, and that lysozyme inhibits the return to the elongation conformation. A kinetic model of pausing and termination is presented that provides a consistent interpretation of our results. | lld:pubmed |
| pubmed-article:9654445 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9654445 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9654445 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9654445 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9654445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9654445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9654445 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9654445 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9654445 | pubmed:issn | 0022-2836 | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:DunnJ JJJ | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:StudierF WFW | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:McAllisterW... | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:FOXJ FJF | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:ZhangXX | lld:pubmed |
| pubmed-article:9654445 | pubmed:author | pubmed-author:LyakhovD LDL | lld:pubmed |
| pubmed-article:9654445 | pubmed:copyrightInfo | Copyright 1998 Academic Press | lld:pubmed |
| pubmed-article:9654445 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9654445 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:9654445 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:9654445 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9654445 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9654445 | pubmed:pagination | 201-13 | lld:pubmed |
| pubmed-article:9654445 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
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| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:meshHeading | pubmed-meshheading:9654445-... | lld:pubmed |
| pubmed-article:9654445 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9654445 | pubmed:articleTitle | Pausing and termination by bacteriophage T7 RNA polymerase. | lld:pubmed |
| pubmed-article:9654445 | pubmed:affiliation | Morse Institute for Molecular Genetics, Health Science Center at Brooklyn, State University of New York, 450 Clarkson Avenue, Brooklyn, Box 44, NY 11203-2098, USA. | lld:pubmed |
| pubmed-article:9654445 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9654445 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9654445 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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