| pubmed-article:9618244 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C1282824 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C0226003 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C0042401 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:9618244 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:9618244 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9618244 | pubmed:dateCreated | 1998-9-17 | lld:pubmed |
| pubmed-article:9618244 | pubmed:abstractText | This study was designed to determine if adenoviral-mediated delivery of a transgene encoding the beta 2-adrenergic receptor (beta 2-AR) to the carotid arterial wall could result in alterations in in vivo vascular function. De-endothelialized rat carotid arteries were infused in vivo with 0.1 mg/ml elastase and adenovirus [6 x 10(9) plaque forming units (PFU)] containing either the marker gene beta-galactosidase (Adeno-beta-gal), DNA encoding the human beta 2-AR (Adeno-beta 2-AR), or no transgene. This low concentration of elastase increased the water permeability (5.2 +/- 0.6 v 1.9 +/- 0.4 x 10(-8) cm/s/mmHg, n = 4, P < 0.0001) without affecting either the vasomotor responsiveness or the morphology of the arterial wall. A transfection efficiency of 73% was achieved with Adeno-beta-gal (n = 3). beta-gal expression was associated with infrequent appearance of T and B lymphocytes, or neutrophil infiltration. Five days after infection with Adeno-beta 2-AR, the total beta-AR density increased six-fold (67.8 +/- 3.4 v 397.0 +/- 155.5 fmol/mg protein, n = 5, P < 0.01); isoproterenol-induced vasorelaxation at transmural pressures from 10-110/mmHg increased (P < 0.01) compared to arteries exposed to control virus (empty adenovirus), n = 4; and isoproterenol-stimulated cAMP production was increased by 65% (n = 5). Thus, adenoviral-mediated delivery of beta 2-ARs into large artery walls results in enhanced beta-AR-mediated vasorelaxation via augmentation in cAMP levels in vascular smooth muscle cells. | lld:pubmed |
| pubmed-article:9618244 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9618244 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9618244 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9618244 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9618244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9618244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9618244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9618244 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9618244 | pubmed:month | May | lld:pubmed |
| pubmed-article:9618244 | pubmed:issn | 0022-2828 | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:LefkowitzR... | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:DolberP CPC | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:GoldmanSS | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:AguirreMM | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:PennerP EPE | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:KochW JWJ | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:PennockG DGD | lld:pubmed |
| pubmed-article:9618244 | pubmed:author | pubmed-author:GaballaM AMA | lld:pubmed |
| pubmed-article:9618244 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9618244 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:9618244 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9618244 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9618244 | pubmed:pagination | 1037-45 | lld:pubmed |
| pubmed-article:9618244 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9618244 | pubmed:meshHeading | pubmed-meshheading:9618244-... | lld:pubmed |
| pubmed-article:9618244 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9618244 | pubmed:articleTitle | Enhanced vasorelaxation by overexpression of beta 2-adrenergic receptors in large arteries. | lld:pubmed |
| pubmed-article:9618244 | pubmed:affiliation | Department of Surgery, Duke University, Durham, NC, USA. | lld:pubmed |
| pubmed-article:9618244 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9618244 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9618244 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:9618244 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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