| pubmed-article:9597545 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9597545 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9597545 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9597545 | pubmed:dateCreated | 1998-8-25 | lld:pubmed |
| pubmed-article:9597545 | pubmed:abstractText | An established lepidopteran insect cell line (Sf9) was cotransfected with expression plasmids encoding neomycin phosphotransferase and bovine beta 1,4-galactosyltransferase. Neomycin-resistant transformants were selected, assayed for beta 1,4-galactosyltransferase activity, and the transformant with the highest level of enzymatic activity was characterized. Southern blots indicated that this transformed Sf9 cell derivative contained multiple copies of the galactosyltransferase-encoding expression plasmid integrated at a single site in its genome. One-step growth curves showed that these cells supported normal levels of baculovirus replication. Baculovirus infection of the transformed cells stimulated beta 1,4-galactosyltransferase activity almost 5-fold by 12 h postinfection. This was followed by a gradual decline in activity, but the infected cells still had about as much activity as uninfected controls as late as 48 h after infection and they were able to produce a beta 1,4-galactosylated virion glycoprotein during infection. Infection of the transformed cells with a conventional recombinant baculovirus expression vector encoding human tissue plasminogen activator also resulted in the production of a galactosylated end-product. These results demonstrate that stable transformation can be used to add a functional mammalian glycosyltransferase to lepidopteran insect cells and extend their N-glycosylation pathway. Furthermore, stably-transformed insect cells can be used as modified hosts for conventional baculovirus expression vectors to produce foreign glycoproteins with "mammalianized" glycans which more closely resemble those produced by higher eucaryotes. | lld:pubmed |
| pubmed-article:9597545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9597545 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9597545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9597545 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9597545 | pubmed:month | May | lld:pubmed |
| pubmed-article:9597545 | pubmed:issn | 0959-6658 | lld:pubmed |
| pubmed-article:9597545 | pubmed:author | pubmed-author:ShaperJ HJH | lld:pubmed |
| pubmed-article:9597545 | pubmed:author | pubmed-author:HollisterJ... | lld:pubmed |
| pubmed-article:9597545 | pubmed:author | pubmed-author:JarvisD LDL | lld:pubmed |
| pubmed-article:9597545 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9597545 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:9597545 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9597545 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9597545 | pubmed:pagination | 473-80 | lld:pubmed |
| pubmed-article:9597545 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:meshHeading | pubmed-meshheading:9597545-... | lld:pubmed |
| pubmed-article:9597545 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9597545 | pubmed:articleTitle | Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. | lld:pubmed |
| pubmed-article:9597545 | pubmed:affiliation | Department of Entomology and Center for Advanced Invertebrate Molecular Sciences, Texas A&M University 77843, USA. | lld:pubmed |
| pubmed-article:9597545 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9597545 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9597545 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9597545 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9597545 | lld:pubmed |
