| pubmed-article:9582224 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C2333134 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0086982 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C1880371 | lld:lifeskim |
| pubmed-article:9582224 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:9582224 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9582224 | pubmed:dateCreated | 1998-8-12 | lld:pubmed |
| pubmed-article:9582224 | pubmed:abstractText | The present study was conducted to assess the intracellular signaling pathways mediated by receptors for ATP, uridine triphosphate (UTP), and 2-methylthio ATP (2-MeSATP), by monitoring patch-clamp currents and intracellular calcium mobilization in cultured rat cortical cerebral neurons. All three agonists evoked potassium currents and increased the intracellular free Ca2+ concentration ([Ca2+]i), and these effects were inhibited by the broad G-protein inhibitor guanosine-5'-O-(2-thiodiphosphate) (GDPbetaS) but not by the Gi/o-protein inhibitor pertussis toxin (PTX). UTP-evoked currents were inhibited by either the phospholipase C inhibitor neomycin or the selective protein kinase C (PKC) inhibitor GF109203X, and the rise in cytosolic Ca2+ was inhibited by either neomycin or the inositol 1,4,5-trisphosphate (IP3) receptor antagonist heparin, indicating that the UTP receptor involved phospholipase C-mediated phosphatidylinositol signaling. In contrast, 2-MeSATP-induced currents and rise in cytosolic Ca2+ were not inhibited by either neomycin, or GF109203X, or heparin. 2-MeSATP elicited single-channel currents in the cell-attached patch-clamp configuration and also in excised patches. The G-protein activator GTP gamma S induced single-channel currents in a fashion that mimicked the effect of 2-MeSATP. These data suggest that 2 MeSATP activated potassium channels by a direct action of G-protein beta gamma subunits and increased [Ca2+]i by a mechanism independent of phospholipase C stimulation and IP3 production. ATP-evoked currents were partially inhibited by either neomycin or GF109203X, although the rise in cytosolic Ca2+ was not affected by these inhibitors. ATP produced single-channel currents with two major classes of the slope conductance (86 and 95 pS) in cell-attached patches, each of which is consistent with that achieved by 2-MeSATP (85 pS) or UTP (96 pS); the currents with the lower conductance were observed in the outside-out patch-clamp configuration. These results indicate that P2 receptors for UTP and 2-MeSATP are linked to a PTX-insensitive G-protein involving different signal transduction pathways and that ATP responses are mediated by both of these P2 receptors. | lld:pubmed |
| pubmed-article:9582224 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9582224 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9582224 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9582224 | pubmed:month | May | lld:pubmed |
| pubmed-article:9582224 | pubmed:issn | 0022-3077 | lld:pubmed |
| pubmed-article:9582224 | pubmed:author | pubmed-author:MoriMM | lld:pubmed |
| pubmed-article:9582224 | pubmed:author | pubmed-author:NishizakiTT | lld:pubmed |
| pubmed-article:9582224 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9582224 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:9582224 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9582224 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9582224 | pubmed:pagination | 2513-21 | lld:pubmed |
| pubmed-article:9582224 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:9582224 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9582224 | pubmed:articleTitle | Diverse signal transduction pathways mediated by endogenous P2 receptors in cultured rat cerebral cortical neurons. | lld:pubmed |
| pubmed-article:9582224 | pubmed:affiliation | Department of Physiology, Kobe University School of Medicine, Chuo-ku, Kobe 650, Japan. | lld:pubmed |
| pubmed-article:9582224 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9582224 | lld:pubmed |
