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pubmed-article:9530560pubmed:abstractTextThe creation of large diverse phage antibody libraries from natural sources relies on primers which are able to amplify as many V genes as possible. All functional germline V genes have recently been catalogued in a database, V BASE [1]. Previously published primer sets are unable to recognise all these V genes.lld:pubmed
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pubmed-article:9530560pubmed:articleTitleA definitive set of oligonucleotide primers for amplifying human V regions.lld:pubmed
pubmed-article:9530560pubmed:affiliationInternational School for Advanced Studies, Trieste, Italy. bradbury@icgeb.trieste.itlld:pubmed
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