| pubmed-article:9478973 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0282587 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0032594 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0017950 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0038769 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0599958 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C1412716 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:9478973 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:9478973 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:9478973 | pubmed:dateCreated | 1998-3-25 | lld:pubmed |
| pubmed-article:9478973 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:abstractText | The HNK-1 carbohydrate is expressed on various adhesion molecules in the nervous system and is suggested to play a role in cell-cell and cell-substratum interactions. Here we describe the isolation and functional expression of a cDNA encoding a human sulfotransferase that synthesizes the HNK-1 carbohydrate epitope. A mutant Chinese hamster ovary cell line, Lec2, which stably expresses human neural cell adhesion molecule (N-CAM) (Lec2-NCAM), was first established. Lec2-NCAM was co-transfected with a human fetal brain cDNA library, a cDNA encoding the rat glucuronyltransferase that forms a precursor of the HNK-1 carbohydrate, and a vector encoding the polyoma large T antigen. The transfected Lec2-NCAM cells expressing the HNK-1 glycan were enriched by fluorescence-activated cell sorting. Sibling selection of recovered plasmids resulted in a cDNA encoding a sulfotransferase, HNK-1ST, that directs the expression of the HNK-1 carbohydrate epitope on the cell surface. The deduced amino acid sequence indicates that the enzyme is a type II membrane protein. Sequence analysis revealed that there is a short amino acid sequence in the presumed catalytic domain, which is highly homologous to the corresponding sequence in other Golgi-associated sulfotransferases so far cloned. The amount of HNK-1ST transcript is high in fetal brain compared with fetal lung, kidney, and liver. Expression of HNK-1ST resulted in the formation of the HNK-1 epitope on N-CAM and a soluble chimeric form of HNK-1ST was shown to add a sulfate group to a precursor, GlcAbeta1-->3Galbeta1-->4GlcNAcbeta1-->R, forming sulfo-->3GlcAbeta1-->3Galbeta1-->4GlcNAcbeta1-->R. The results combined together indicate that the cloned HNK-1ST directs the synthesis of the HNK-1 carbohydrate epitope on both glycoproteins and glycolipids in the nervous tissues. | lld:pubmed |
| pubmed-article:9478973 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9478973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9478973 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9478973 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9478973 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:9478973 | pubmed:author | pubmed-author:FukudaMM | lld:pubmed |
| pubmed-article:9478973 | pubmed:author | pubmed-author:HindsgaulOO | lld:pubmed |
| pubmed-article:9478973 | pubmed:author | pubmed-author:DingYY | lld:pubmed |
| pubmed-article:9478973 | pubmed:author | pubmed-author:LUT PTP | lld:pubmed |
| pubmed-article:9478973 | pubmed:author | pubmed-author:YehJ CJC | lld:pubmed |
| pubmed-article:9478973 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9478973 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:9478973 | pubmed:volume | 273 | lld:pubmed |
| pubmed-article:9478973 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9478973 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9478973 | pubmed:pagination | 5190-5 | lld:pubmed |
| pubmed-article:9478973 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9478973 | pubmed:meshHeading | pubmed-meshheading:9478973-... | lld:pubmed |
| pubmed-article:9478973 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9478973 | pubmed:articleTitle | Expression cloning of a human sulfotransferase that directs the synthesis of the HNK-1 glycan on the neural cell adhesion molecule and glycolipids. | lld:pubmed |
| pubmed-article:9478973 | pubmed:affiliation | Glycobiology Program, La Jolla Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA. | lld:pubmed |
| pubmed-article:9478973 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9478973 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:9478973 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9478973 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:9486 | entrezgene:pubmed | pubmed-article:9478973 | lld:entrezgene |
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