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pubmed-article:9475037pubmed:dateCreated1998-3-19lld:pubmed
pubmed-article:9475037pubmed:abstractTextCapacitation of buffalo sperm was evaluated by induced acrosome reaction (AR) upon the exposure of 10 mM Ca2+. Culture of sperm for 8 hr in BO medium supplemented with 10 micrograms/ml heparin significantly (P < 0.01) increased the percentage of AR and confirmed by transmission electron microscopy. Vesiculization of outer acrosomal membrane and plasma membrane was observed significantly higher (P < 0.01) following 8 hr of sperm culture with heparin. Culture of sperm with heparin also increased rate of fertilization of in vitro matured oocytes and their subsequent development up to morula/blastocyst stage (P < 0.01). The study demonstrates that capacitation of buffalo sperm by heparin required at least 8 hr exposure of sperm to heparin for maximum acrosome reaction.lld:pubmed
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pubmed-article:9475037pubmed:authorpubmed-author:ChauhanM SMSlld:pubmed
pubmed-article:9475037pubmed:authorpubmed-author:MadanM LMLlld:pubmed
pubmed-article:9475037pubmed:authorpubmed-author:SinglaS KSKlld:pubmed
pubmed-article:9475037pubmed:authorpubmed-author:ManikR SRSlld:pubmed
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pubmed-article:9475037pubmed:volume35lld:pubmed
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pubmed-article:9475037pubmed:pagination1038-43lld:pubmed
pubmed-article:9475037pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:9475037pubmed:year1997lld:pubmed
pubmed-article:9475037pubmed:articleTitleIncreased capacitation of buffalo sperm by heparin as confirmed by electron microscopy and in vitro fertilization.lld:pubmed
pubmed-article:9475037pubmed:affiliationEmbryo Biotechnology Centre, National Dairy Research Institute, Karnal, India.lld:pubmed
pubmed-article:9475037pubmed:publicationTypeJournal Articlelld:pubmed