Linked Life Data

9438449

Source:http://linkedlifedata.com/resource/pubmed/id/9438449

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pubmed-article:9438449pubmed:abstractTextA combination of 31P and 23Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na]IC in the isolated and perfused mouse liver. The 31P resonances of dimethyl methylphosphonate and LaDOTP5-, markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 +/- 0.3 g, the intracellular liver volume as measured by 31P NMR averaged 1.2 +/- 0.2 ml. The amount of intracellular sodium was measured from the baseline-resolved intracellular 23Na resonance during perfusion of the shift reagent, TmDOTP5-. These two measurements resulted in an NMR-determined value for [Na]IC of 29.0 +/- 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS-determined value for [Na]IC of 32.1 +/- 7.4 mM. These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23Na NMR spectroscopy.lld:pubmed
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pubmed-article:9438449pubmed:authorpubmed-author:SherryA DADlld:pubmed
pubmed-article:9438449pubmed:authorpubmed-author:ColesJ LJLlld:pubmed
pubmed-article:9438449pubmed:authorpubmed-author:MalloyC RCRlld:pubmed
pubmed-article:9438449pubmed:authorpubmed-author:MakosJ DJDlld:pubmed
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pubmed-article:9438449pubmed:pagination155-9lld:pubmed
pubmed-article:9438449pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:9438449pubmed:year1998lld:pubmed
pubmed-article:9438449pubmed:articleTitleDetermination of the intracellular sodium concentration in perfused mouse liver by 31P and 23Na magnetic resonance spectroscopy.lld:pubmed
pubmed-article:9438449pubmed:affiliationDepartment of Chemistry, University of Texas at Dallas, Richardson, USA.lld:pubmed
pubmed-article:9438449pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9438449pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:9438449pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:9438449pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed