| pubmed-article:9346294 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C0678640 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:9346294 | lifeskim:mentions | umls-concept:C0074995 | lld:lifeskim |
| pubmed-article:9346294 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9346294 | pubmed:dateCreated | 1997-11-21 | lld:pubmed |
| pubmed-article:9346294 | pubmed:abstractText | The effect of sphingosylphosphorylcholine (SphPCho) on the intracellular pH (pHi) in GH4C1 cells was investigated. SphPCho evoked a very slow increase in basal pHi. In cells acidified with nigericin, SphPCho induced a rapid alkalinization of the cells. The effect was inhibited in a Na+-free buffer solution, but was insensitive to ethylisopropyl amiloride, a potent inhibitor of Na+-H+ exchangers (NHE). Reverse transcription and PCR showed that the predominant isoform of the antiport expressed in GH4C1 cells is NHE-1. The rate of alkalinization after stimulation with propionate, and after addition of Na+ to cells acidified with NH4Cl, was enhanced in cells treated with SphPCho. The initial rate of alkalinization after addition of Na+ to acidified cells treated with SphPCho gave an apparent Km value of 15 +/- 2 mM for Na+. The Vmax value was 9 +/- 2 mM H+/min. The effect was insensitive to ouabain, staurosporine and bafilomycin A. However, the SphPCho-evoked alkalinization was abolished in cells treated with 2-deoxy-D-glucose. The effect was not due to the charge of the molecule, as stearylamine increased pHi in Na+-containing and Na+-free buffer. The results show that SphPCho may activate Na+-H+ exchange, and that this effect is mediated via an amiloride-insensitive exchange mechanism. | lld:pubmed |
| pubmed-article:9346294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9346294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9346294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9346294 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9346294 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:9346294 | pubmed:author | pubmed-author:GrinsteinSS | lld:pubmed |
| pubmed-article:9346294 | pubmed:author | pubmed-author:WoodsideMM | lld:pubmed |
| pubmed-article:9346294 | pubmed:author | pubmed-author:TörnquistKK | lld:pubmed |
| pubmed-article:9346294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9346294 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9346294 | pubmed:volume | 248 | lld:pubmed |
| pubmed-article:9346294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9346294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9346294 | pubmed:pagination | 394-400 | lld:pubmed |
| pubmed-article:9346294 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:9346294 | pubmed:meshHeading | pubmed-meshheading:9346294-... | lld:pubmed |
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| pubmed-article:9346294 | pubmed:meshHeading | pubmed-meshheading:9346294-... | lld:pubmed |
| pubmed-article:9346294 | pubmed:meshHeading | pubmed-meshheading:9346294-... | lld:pubmed |
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| pubmed-article:9346294 | pubmed:meshHeading | pubmed-meshheading:9346294-... | lld:pubmed |
| pubmed-article:9346294 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9346294 | pubmed:articleTitle | Sphingosylphosphorylcholine activates an amiloride-insensitive Na+-H+-exchange mechanism in GH4C1 cells. | lld:pubmed |
| pubmed-article:9346294 | pubmed:affiliation | Department of Biosciences, University of Helsinki, Finland. | lld:pubmed |
| pubmed-article:9346294 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9346294 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
