| pubmed-article:9284914 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9284914 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:9284914 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:9284914 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:9284914 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:9284914 | pubmed:dateCreated | 1997-9-25 | lld:pubmed |
| pubmed-article:9284914 | pubmed:abstractText | K562 is a cell line with two acrocentric marker chromosomes containing abnormally banded regions (ABRs), derived from a Ph-positive chronic myelogenous leukemia (CML) patient. Using reverse and forward chromosome painting FISH analysis, we found that 9q34, 13q31, and 22q11 regions co-amplified in the ABRs-bearing acrocentric marker chromosomes of K562. Utilizing the ABRs of the cell line as target DNA for cDNA selection, we established a simple procedure for chromosome region-specific cDNA isolation. After first strand cDNA synthesis from fetal brain mRNAs, short fragment cDNAs (sf-cDNAs) were synthesized with a two-step amplification system by use of our modified Degenerate Oligonucleotide Primed Shuttle Polymerase Chain Reaction (DOP-Shuttle-PCR) method. The sf-cDNAs were hybridized onto RNase A treated metaphases from K562, and the ABRs were microdissected and reamplified with DOP-Shuttle-PCR primer-II. The reamplified sf-cDNAs were cloned into a pBluescript vector. Twenty randomly chosen clones were sequenced and classified into 8 groups. Three out of the 8 grouped clones had been mapped to the long arm of chromosome 22 (22q11), whereas the other 5 were novel cDNAs. Quantitative Southern blot analysis indicated that 7 out of the 8 grouped clones (87.5%) were derived from the co-amplified regions. | lld:pubmed |
| pubmed-article:9284914 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9284914 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9284914 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9284914 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9284914 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9284914 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9284914 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9284914 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9284914 | pubmed:issn | 0301-0171 | lld:pubmed |
| pubmed-article:9284914 | pubmed:author | pubmed-author:YokoyamaYY | lld:pubmed |
| pubmed-article:9284914 | pubmed:author | pubmed-author:SakuragawaNN | lld:pubmed |
| pubmed-article:9284914 | pubmed:author | pubmed-author:KozakiTT | lld:pubmed |
| pubmed-article:9284914 | pubmed:author | pubmed-author:OhsugiKK | lld:pubmed |
| pubmed-article:9284914 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9284914 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:9284914 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9284914 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9284914 | pubmed:pagination | 192-6 | lld:pubmed |
| pubmed-article:9284914 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:meshHeading | pubmed-meshheading:9284914-... | lld:pubmed |
| pubmed-article:9284914 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9284914 | pubmed:articleTitle | Microdissection-mediated selection of chromosome region-specific cDNAs. | lld:pubmed |
| pubmed-article:9284914 | pubmed:affiliation | Center for Molecular Biology and Cytogenetics SRL, Inc., Hachioji, Tokyo, Japan. | lld:pubmed |
| pubmed-article:9284914 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9284914 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9284914 | lld:pubmed |
