| pubmed-article:9257904 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C0034650 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C2339371 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C0205464 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:9257904 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:9257904 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:9257904 | pubmed:dateCreated | 1997-9-29 | lld:pubmed |
| pubmed-article:9257904 | pubmed:abstractText | 1. The whole-cell patch-clamp was used for studying the effects of various beta1- and beta2-adrenoceptor agonists and antagonists on the L-type Ca current (Ica) in frog ventricular myocytes. 2. Dose-response curves for the effects of isoprenaline (non selective beta-agonist), salbutamol (beta2-agonist), dobutamine (beta1-agonist) on ICa were obtained in the absence and presence of various concentrations of ICI 118551 (beta2-antagonist), metoprolol (beta1-antagonist) and xamoterol (partial beta1-agonist) to derive EC50 (i.e. the concentration of beta-agonist at which the response was 50% of the maximum) and Emax (the maximal response) values by use of a Michaelis equation. Schild regression analysis was performed to examine whether the antagonists were competitive and to determine the equilibrium dissociation constant (K(B)) for the antagonist-receptor complex. 3. Isoprenaline increased ICa with an EC50 of 20.0 nM and an Emax of 597%. ICI 118551 and metoprolol competitively antagonized the effect of isoprenaline with a K(B) of 3.80 nM and 207 nM, respectively. 4. Salbutamol increased ICa with an EC50 of 290 nM and an Emax of 512%. ICI 118551 and metoprolol competitively antagonized the effect of salbutamol with a K(B) of 1.77 nM and 456 nM, respectively. 5. Dobutamine increased ICa with an EC50 of 2.40 microM and an Emax of 265%. ICI 118551 and metoprolol competitively antagonized the effect of dobutamine with a K(B) of 2.84 nM and 609 nM, respectively. 6. Xamoterol had no stimulating effect on ICa. However, xamoterol competitively antagonized the stimulating effects of isoprenaline, salbutamol and dobutamine on ICa with a K(B) of 58-64 nM. 7. We conclude that a single population of receptors is involved in the beta-adrenoceptor-mediated regulation of ICa in frog ventricular myocytes. The pharmacological pattern of the response of ICa to the different beta-adrenoceptor agonists and antagonists tested suggests that these receptors are of the beta2-subtype. | lld:pubmed |
| pubmed-article:9257904 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9257904 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9257904 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9257904 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9257904 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9257904 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9257904 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9257904 | pubmed:issn | 0007-1188 | lld:pubmed |
| pubmed-article:9257904 | pubmed:author | pubmed-author:FischmeisterR... | lld:pubmed |
| pubmed-article:9257904 | pubmed:author | pubmed-author:JureviciusJJ | lld:pubmed |
| pubmed-article:9257904 | pubmed:author | pubmed-author:SkeberdisV... | lld:pubmed |
| pubmed-article:9257904 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9257904 | pubmed:volume | 121 | lld:pubmed |
| pubmed-article:9257904 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9257904 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9257904 | pubmed:pagination | 1277-86 | lld:pubmed |
| pubmed-article:9257904 | pubmed:dateRevised | 2008-11-20 | lld:pubmed |
| pubmed-article:9257904 | pubmed:meshHeading | pubmed-meshheading:9257904-... | lld:pubmed |
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| pubmed-article:9257904 | pubmed:meshHeading | pubmed-meshheading:9257904-... | lld:pubmed |
| pubmed-article:9257904 | pubmed:meshHeading | pubmed-meshheading:9257904-... | lld:pubmed |
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| pubmed-article:9257904 | pubmed:meshHeading | pubmed-meshheading:9257904-... | lld:pubmed |
| pubmed-article:9257904 | pubmed:meshHeading | pubmed-meshheading:9257904-... | lld:pubmed |
| pubmed-article:9257904 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9257904 | pubmed:articleTitle | Pharmacological characterization of the receptors involved in the beta-adrenoceptor-mediated stimulation of the L-type Ca2+ current in frog ventricular myocytes. | lld:pubmed |
| pubmed-article:9257904 | pubmed:affiliation | Laboratoire de Cardiologie Cellulaire et Moléculaire, INSERM U-446, Université de Paris-Sud, Faculté de Pharmacie, Chatênay-Malabry, France. | lld:pubmed |
| pubmed-article:9257904 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9257904 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:9257904 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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