| pubmed-article:9219895 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0009235 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0597763 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0032172 | lld:lifeskim |
| pubmed-article:9219895 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9219895 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9219895 | pubmed:dateCreated | 1997-8-7 | lld:pubmed |
| pubmed-article:9219895 | pubmed:abstractText | The regulation of PAF synthesis by the macrophage cell line IC-21 challenged with bacterial endotoxin was investigated. The LPS-induced increase in cellular PAF levels was rapid, sustained and attained maximal levels within 30 min following LPS stimulation. PAF accumulation was accompanied by the activation of the CoA-independent transacylase and acetyl-CoA: lyso-PAF acetyltransferase, whereas the release of free [3H]arachidonic acid in prelabeled cells reflecting the activation of phospholipase A2, occurred primarily within the initial 1-5 min of treatment with LPS. Cell lysates from LPS-stimulated macrophages exhibited a markedly increased enzymatic activity that was capable of both acylation of 1-[3H]alkyl-2-lyso-GPC (lyso-PAF) and deacylation of 1-[3H]alkyl-2-acyl-GPC generating [3H]lyso-PAF via CoA-independent transacylation of exogenous lysoplasmenylethanolamine compared with extracts from resting macrophages. Pretreatment of the cells with LPS for 5 and 30 min enhanced significantly the transfer of [14C]arachidonic acid from 1-[3H]alkyl-2-[14C]arachidonoyl-GPC into plasmenylethanolamine in prelabeled cell homogenates following the addition of exogenous lysoplasmenylethanolamine. Taken together, these data suggest that the CoA-independent transacylase, but not phospholipase A2, is a key enzyme responsible for the prolonged generation of lyso-PAF and that the increased capability of CoA-independent transacylation followed by CoA-dependent acetylation of lyso-PAF can sustain the biosynthesis of PAF in LPS-stimulated IC-21 macrophages. | lld:pubmed |
| pubmed-article:9219895 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9219895 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9219895 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9219895 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9219895 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:9219895 | pubmed:author | pubmed-author:LiuHH | lld:pubmed |
| pubmed-article:9219895 | pubmed:author | pubmed-author:ChanVV | lld:pubmed |
| pubmed-article:9219895 | pubmed:author | pubmed-author:OlsonM SMS | lld:pubmed |
| pubmed-article:9219895 | pubmed:author | pubmed-author:SvetlovS ISI | lld:pubmed |
| pubmed-article:9219895 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9219895 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:9219895 | pubmed:volume | 1346 | lld:pubmed |
| pubmed-article:9219895 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9219895 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9219895 | pubmed:pagination | 120-30 | lld:pubmed |
| pubmed-article:9219895 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:meshHeading | pubmed-meshheading:9219895-... | lld:pubmed |
| pubmed-article:9219895 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9219895 | pubmed:articleTitle | Regulation of platelet-activating factor (PAF) biosynthesis via coenzyme A-independent transacylase in the macrophage cell line IC-21 stimulated with lipopolysaccharide. | lld:pubmed |
| pubmed-article:9219895 | pubmed:affiliation | Department of Biochemistry, The University of Texas Health Science Center, San Antonio 78284-7760, USA. | lld:pubmed |
| pubmed-article:9219895 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9219895 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
