| pubmed-article:9217192 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C0006142 | lld:lifeskim |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C1518999 | lld:lifeskim |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C0040736 | lld:lifeskim |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:9217192 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:9217192 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9217192 | pubmed:dateCreated | 1997-7-23 | lld:pubmed |
| pubmed-article:9217192 | pubmed:abstractText | Contamination of transplants with tumour cells may contribute to relapse after peripheral blood stem cell transplantation (PBSCT). We studied the feasibility of CD34+ cell selection from blood-derived autografts obtained following G-CSF-supported cytotoxic chemotherapy in a group of 25 patients with breast cancer (10 with high-risk stage II/III and 15 with stage IV without bone or bone marrow involvement). Using immunomagnetic beads (Isolex 300 SA. Baxter) CD34+ cells were enriched and released by chymopapain resulting in a median purity of 95% (range 82-99%) and a median recovery of 80% (range 27-132%). The enrichment procedure did not change the proportion of CD34+ subsets coexpressing HLA-DR, CD38 and Thy-1, while L-selectin was removed from the cell surface following selection. Using a sensitive immunocytological technique with a cocktail of epithelial-specific antibodies (anti-cytokeratin 8, 18 and 19; HEA125; BM7 and BM8), five leukaphereses products contained epithelial cells, whereas the selected CD34+ cell fraction was free of tumour cells. A neutrophil count of 0.5 x 10(9)/l and a platelet count of 20 x 10(9)/l was reached after a median time of 14 and 10d following 40 high-dose chemotherapy (HDC) cycles. Our results indicate that immunomagnetic selection of CD34+ cells yields highly purified autografts devoid of tumour cells whereas the engraftment ability of the progenitor and stem cells is fully retained. | lld:pubmed |
| pubmed-article:9217192 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9217192 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9217192 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9217192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9217192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9217192 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9217192 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9217192 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9217192 | pubmed:issn | 0007-1048 | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:HaasRR | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:SchmidHH | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:KaulSS | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:LiuY LYL | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:AbdallahAA | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:FungHH | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:MurerBB | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:HohaudBB | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:WallwienerDD | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:VosoM TMT | lld:pubmed |
| pubmed-article:9217192 | pubmed:author | pubmed-author:PförsichMM | lld:pubmed |
| pubmed-article:9217192 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9217192 | pubmed:volume | 97 | lld:pubmed |
| pubmed-article:9217192 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9217192 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9217192 | pubmed:pagination | 881-8 | lld:pubmed |
| pubmed-article:9217192 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:meshHeading | pubmed-meshheading:9217192-... | lld:pubmed |
| pubmed-article:9217192 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9217192 | pubmed:articleTitle | Immunomagnetic selection of CD34+ peripheral blood stem cells for autografting in patients with breast cancer. | lld:pubmed |
| pubmed-article:9217192 | pubmed:affiliation | Department of Internal Medicine V, University of Heidelberg, Germany. | lld:pubmed |
| pubmed-article:9217192 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9217192 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9217192 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9217192 | lld:pubmed |
